FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252536878
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119733104.741212489369572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3979660.1575872811732471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41156400.091.142539
GGTATCA41347100.090.774088
AGTGGTA41457150.090.64835
GTGGTAT41431700.090.5605856
AAGCAGT41822950.090.226971
TGGTATC41630750.090.056747
AGCAGTG42605900.088.554222
CAGTGGT42828200.087.7849044
GCAGTGG43565400.086.549043
CAACGCA40662000.046.52639812-13
ATCAACG40822600.046.29064610-11
TCAACGC40649200.046.25194512-13
ACGCAGA41060400.046.12113614-15
AACGCAG41201150.045.68663414-15
AGAGTAC40565250.045.62085718-19
CGCAGAG41448700.045.34490616-17
TATCAAC41510700.045.25078210-11
GCAGAGT41552650.045.18604316-17
CAGAGTA40947200.045.11401418-19
GAGTACA26024000.044.57964320-21
Produced by FastQC (version 0.11.5)