FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245122405
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64283612.6225105779294227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20876950.089.002529
GTGGTAT20940350.088.6855556
GGTATCA21069900.088.196478
TGGTATC21178050.087.539937
AGTGGTA21372500.086.9031455
AAGCAGT21658300.086.140241
AGCAGTG22588750.082.6299062
CAGTGGT22619800.082.140834
GCAGTGG24087150.077.4755253
CAACGCA20690950.045.37384812-13
ATCAACG20786450.045.24242410-11
TCAACGC20701850.045.049612-13
ACGCAGA21078250.044.61174814-15
AACGCAG21065000.044.31915314-15
AGAGTAC20818600.044.17506418-19
TATCAAC21229800.043.84501310-11
CGCAGAG21283100.043.82445516-17
CAGAGTA21186500.043.3830918-19
GCAGAGT21602050.043.250716-17
GAGTACA13263300.042.23185720-21
Produced by FastQC (version 0.11.5)