FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239285880
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1570150.022.7086729
GGTATCA1635650.021.8061568
CAACGCA1042850.020.92843212-13
ATCAACG1082400.020.3666110-11
GTGGTAT1753850.020.360526
TGGTATC1874250.019.0874887
TCAACGC971950.018.50302512-13
AGTGGTA1971850.018.2409295
ACGCAGA1294200.017.11300714-15
AAGCAGT2664000.014.1468511
AACGCAG1354150.013.48426814-15
AGAGTAC1683300.013.109138518-19
AGCAGTG2916300.012.8752562
CGCAGAG1433550.012.71162316-17
CAGTGGT3153800.011.9352874
TATCAAC1773150.010.68002310-11
GATCGGA319900.010.6421651
GCAGAGT2168750.010.45696616-17
GAGCGGT357200.09.5634429
GAGTACT1564000.09.35386420-21
Produced by FastQC (version 0.11.5)