FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003623590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003623590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214505551
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2649940.12353712934916077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1384250.027.751899
GGTATCA1671900.023.0171558
CAACGCA1006150.022.76444612-13
GTGGTAT1726250.022.7555126
AGTGGTA1838000.021.4621125
TCAACGC947800.020.67041612-13
TGGTATC1908700.020.5267357
ATCAACG1113600.020.33730910-11
ACGCAGA1298700.017.88326514-15
AAGCAGT2365150.016.945641
AGAGTAC1533300.014.98104818-19
AACGCAG1383100.014.49765314-15
AGCAGTG2827300.014.188542
CGCAGAG1486950.013.31000316-17
CAGTGGT3052100.013.2493234
GCAGAGT2018850.011.61928516-17
TATCAAC1739900.011.51866510-11
GCAGTGG3592650.011.2773723
CAGAGTA1924150.010.39722718-19
GAGTACT1476500.010.06833220-21
Produced by FastQC (version 0.11.5)