FastQCFastQC Report
Tue 10 May 2022
EGAF00003624250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728777
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC105111.4422793255001187No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG93341.280775875199135No Hit
GTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGC44140.6056722426750569No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41130.5643701708478726No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT40480.5554511187921682No Hit
GTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGT39450.5413178516885138No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT32070.44005230680990204No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA30680.4209792570292421No Hit
GTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT26410.36238794583253864No Hit
TATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTC25800.35401775851872386No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT25710.35278281284947244No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA23230.3187531988523238No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT22640.3106574439094538No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT21960.3013267432973324No Hit
ACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC21360.29309377216898996No Hit
GAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA20930.2871934761936779No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC20840.28595853052442655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19040.26125961713939927No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT18580.2549476726076701No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG17320.23765843323815106No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG17150.2353257580851207No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG16840.2310720563354771No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT15720.21570384356257127No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC14750.202393873571751No Hit
GGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACG14470.19855182037852456No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA13350.18318360760561872No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC13170.18071371626711602No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG13080.17947877059786463No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC12430.17055971854216034No Hit
ACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT12050.16534550349421015No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG11560.15862191040606385No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT11250.15436820865642029No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG10950.15025172309224907No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC10770.14778183175374635No Hit
GTACATGGGCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA10490.14393977856051987No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC10320.14160710340748953No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG10170.13954886062540392No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT9830.13488351031934323No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG9730.13351134846461948No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG9640.13227640279536815No Hit
GTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGC9320.1278854848602522No Hit
GGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT9290.12747383630383505No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG9250.12692497156194557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.12541559352174944No Hit
GTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTC9100.12486672877985996No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG9010.12363178311060859No Hit
CATGGGAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTT8920.12239683744135724No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC8670.1189664328045479No Hit
GTACATGGGCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT8630.1184175680626584No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA8480.1163593252805728No Hit
CCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG8470.11622210909510043No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC8390.11512437961132144No Hit
CATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT7810.10716584085392376No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT7680.10538203044278291No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG7450.10222605817691832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)