FastQCFastQC Report
Tue 10 May 2022
EGAF00003624304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81067
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10141.2508172252581198No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4850.5982705663216845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4180.5156228798401322No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3600.4440771213934153No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2780.3429262215204707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2660.3281236508073569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.2898836764651461No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2260.2787817484303107No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2130.26274563015777075Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1800.22203856069670766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.22080501313728151No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT1780.21957146557785534No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1770.2183379180184292No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1700.20970308510244612No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1640.20230179974588922Clontech SMART CDS Primer II A (100% over 26bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1630.20106825218646307No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1530.1887327765922015Clontech Universal Primer Mix Long (96% over 26bp)
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1470.1813314912356446No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1350.16652892052253074No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1320.1628282778442523No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1270.15666054004712152Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1220.15049280224999076No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1170.14432506445285997Clontech SMART CDS Primer II A (100% over 26bp)
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG1130.13939087421515536No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1060.1307560412991723No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1040.12828894618031997No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT1040.12828894618031997No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT1000.12335475594261536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.12212120838318921No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA970.11965411326433692No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC960.11842056570491076Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT950.11718701814548459No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG940.11595347058605844No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT940.11595347058605844No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT930.1147199230266323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG930.1147199230266323No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA920.11348637546720615No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA900.11101928034835383No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC870.10731863767007538No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC860.1060850901106492No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC830.10238444743237075No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC820.10115089987294461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)