FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003624478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGC32.272727272727273No Hit
GTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTG32.272727272727273No Hit
TTGTGGCACCGTGATCCAGTATATGAGCTACCGAGTCATTCTGATAGATC32.272727272727273No Hit
CCATGCCCCAAACAGGTTTGAACACAAATCTTTCGGATGAAAATTAGAGA32.272727272727273No Hit
ACTCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGA32.272727272727273No Hit
GGTCTTATTATCCTGCCTAGGCTGGTCTTGAATGCCTAGCCTCAATATCC32.272727272727273No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT21.5151515151515151No Hit
CGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACT21.5151515151515151No Hit
GGACCGCGGTTCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCG21.5151515151515151No Hit
CTCCTGGGGTGTGCTGGCCTCCCTGGCCTACCAGCGCTACAAGGCTGGCG21.5151515151515151No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA21.5151515151515151No Hit
GTGTATCCAGGGTGCTCTGTTTCCCCACCGTTCCCAGGTGTACGAGGCAG21.5151515151515151No Hit
TGTTTGAGATGGCAATTCTTATGTTAGCCCACAAGCGTATTTTTACCTGG21.5151515151515151No Hit
GTATCAACGCAGAGTACATGGGGGGAGAGGAGAGCATAGCACCTGCAGCA21.5151515151515151No Hit
AGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACG21.5151515151515151No Hit
GGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGT21.5151515151515151No Hit
CGCGTGCTGGACATACTGGACCAGTACGGTGCCAAGGCCACCTTCTTCTG21.5151515151515151No Hit
GATCAGCGGGTCAAGGGGCCAGGCGTCGCCGTCGGTCAGGAAGGCGGGCG21.5151515151515151No Hit
TAGTTGAGTGGAGTAGCTATTCATGAGGCTAGGAAGTTTATCTGGAGGCA21.5151515151515151No Hit
CTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTA21.5151515151515151No Hit
CCGCGGGTCATAGAGAAAGCGCCGCATGATCCGGCCATCGCGCCCATGGA21.5151515151515151No Hit
CCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCG21.5151515151515151No Hit
GGGCCGGCGGGGTCAGTACTCCACTGATCCTGCCCATGTGCCCCAGAAAC21.5151515151515151No Hit
AGTTTATTGAGTAAAAAAATGCATACATTGGAACGGCAAAACATCAATAA21.5151515151515151No Hit
CACTTCGGGAGGCCAAGGCAGGTAGATGGCTGTGACTAGCCTGGGCAACA21.5151515151515151No Hit
CTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTAC10.7575757575757576No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC10.7575757575757576No Hit
CAGCAACCCGGCGTTGGCGAACACAAGAAGGATGGCCAGGCCCAGCACGA10.7575757575757576No Hit
CTGCATTGTGGAGTGCAGGCCGTCTGGCGCGAGGCGGCGGGCGCGTTCTG10.7575757575757576No Hit
GGCCTGAAGCTAGTGGATAAGTGTTTGGAGGATGTTAAAAAGTTGTACCA10.7575757575757576No Hit
GTGTAACGGGAACGCTGCAGCCACTGGGCATTGGGTGGTCAGCCGGGGTA10.7575757575757576No Hit
GTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGA10.7575757575757576No Hit
GTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAG10.7575757575757576No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10.7575757575757576No Hit
CTGTTAGCCATAAGCACTTTACAAACAGACTCAGGAAACTATGCAGTAGA10.7575757575757576No Hit
TTTATAATTATTATAAAGTTGAACCGCTGAAACTTGTTCACTGAAACATT10.7575757575757576No Hit
CACACGCTGGGGCTGCTGTCACAGGACAATCTTAAAGGAGCTGGCAAAGT10.7575757575757576No Hit
CTGCTGCACTTGACCAGCTCCATGAACTCGGAGAAGAGAAAATATCAACT10.7575757575757576No Hit
GGGGTTGGTGTTAATTGTCCTTTGAAGTATCCTTGTGGAGGGGGACGGGC10.7575757575757576No Hit
AGTTACCACAGGGCTAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACG10.7575757575757576No Hit
CTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAG10.7575757575757576No Hit
TCACAGAGCCGTTATTCCCCGAGGGGGAGAAACTGCCATTGGTGAGAGCT10.7575757575757576No Hit
GCCCCTACCAGCATCAGGAATACCTGGTCTTCGTTCATGTCAAGAACATT10.7575757575757576No Hit
CAGGAGAAGATGGCTGCTTTCACTTCCCCCCATTGAGCTCTGCTCCCTCT10.7575757575757576No Hit
CCCGTGTGGCACGCAGCACCCACCTGGTCGCGCTGCTCGTGCAGGTCGAC10.7575757575757576No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG10.7575757575757576No Hit
GTCCACCAGTGGAAGTGACTGCCGAGGAAGGGCGGTGAGGAGGGCGGTGT10.7575757575757576No Hit
GATTACAGGCATTAGCCACTGTGCCCGGCCTATATATATACTTTTCTATC10.7575757575757576No Hit
GTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAG10.7575757575757576No Hit
GCCTATTGGCTGGAGGGAGAGGCTTAGGTAGAAGCCCTATTACTTTGCAA10.7575757575757576No Hit
TTTGGATGCTATGCATTTCATTTTTAATTTCCTAGTCACTTCTTTTGAAA10.7575757575757576No Hit
CTCCAGTGGAGCATAAGGGCAAAAGCTCGCTTGATCTTGATATTAATTAA10.7575757575757576No Hit
AAATCAATCAACAAAAAACACAACCAAAAAAAAACACCAAAAACAGACAA10.7575757575757576No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAATTCCTTTA10.7575757575757576No Hit
ACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGT10.7575757575757576No Hit
AGATTGGAGGGTGGAACAGATTTTTGATAACTTAGTGAGATAACATGAAA10.7575757575757576No Hit
GTCATGGACGGCAACGGACGTTGGGCCAGGCAACGCGGTCTTAGGCGCAC10.7575757575757576No Hit
GGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTTAATCACT10.7575757575757576No Hit
ATTGAATGTTGAATAATCATGATGTAGAAAGCACTGTGGGAGGGTGGTAT10.7575757575757576No Hit
TCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC10.7575757575757576No Hit
GAAAGGCAGTTACACTGAAGAACTTAACTTAGAATAAAATGGAAGCAAAC10.7575757575757576No Hit
CACCCAGGTGATGAACTGGTCGTCGACTCCACCAGCAGTGATGAGGTCAC10.7575757575757576No Hit
GATGAACATCGCAGCGCCCTGGTCGGGCTACGAGCAGGAGTCTCTCAACA10.7575757575757576No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAG10.7575757575757576No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10.7575757575757576No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG10.7575757575757576No Hit
GTGTTGCAGCAAACCCTGGTGACCGCCATCGAACTGCTCTCGCCTGCCAA10.7575757575757576No Hit
CATCTTCTCAGTCAATTGTTTCACATGTTCCTGATAACTCTTCTCTTTCT10.7575757575757576No Hit
GCGATGATGCGTGAGGCGGCGGCGCGCGGCTATGCCATCTACACGTGCCT10.7575757575757576No Hit
GTACTTAAGCCATGCGTACTTAGACATGCATGGCTTAATCTTTGAGACAA10.7575757575757576No Hit
ATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGG10.7575757575757576No Hit
CACTTAATGATTGTTGGCAAGAAATGTGCTGCTGATCTGGGCCTGAATAA10.7575757575757576No Hit
CTGAAGTGCTTCTTCCTTCCTGGATGAGTGCAGGGAGAGGCAGGACAGGG10.7575757575757576No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT10.7575757575757576No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT10.7575757575757576No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG10.7575757575757576No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG10.7575757575757576No Hit
GTTTTGGCCAAGCTGGTCTTGAACTCCTGACCTCAGGGGATCCACCCATC10.7575757575757576No Hit
GTTCGGCGAAGAAGGACATCAAGAAGGTCCTCGCCGGATCGACGATGAGC10.7575757575757576No Hit
GTTCTAACTTCCACCAGGCAACCAACTTTGCAATCCACTAACTTTCTTTC10.7575757575757576No Hit
CCCTCACTGATGTCCCGGGTGGCCGAGTCGGTGCAGGTGGAGGAAGAAGA10.7575757575757576No Hit
ATAAGGGTGTATTTGATTGAGATAAGTATTCATTAGAAATAAATTTCAAA10.7575757575757576No Hit
CCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAA10.7575757575757576No Hit
GAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGC10.7575757575757576No Hit
GGCCTGAAGCTAGTGGATAAGTTTTTGGAGGATGTTAAAAAGTTGTACCA10.7575757575757576No Hit
GGAGAGGCCTCGCCCCCCGAGAGGTGCCGCCAGCTGTTGGGCTGCTGCTG10.7575757575757576No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7575757575757576Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC10.7575757575757576No Hit
TCCCAATTCAACGCCCAGGCGCTCCTGCTCGCCTCCAGCCACGCCCTCGG10.7575757575757576No Hit
GATTGAATAGTACATAATATGCTGTATTAGAAGTGCTTGTCAAGGTTCTT10.7575757575757576No Hit
AAATTGGCTGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAG10.7575757575757576No Hit
TGATAACTTGGCCAATGTGAACCCTGGCCACAGTGGCCAGGGTTCACATT10.7575757575757576No Hit
TCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG10.7575757575757576No Hit
GTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC10.7575757575757576No Hit
GTGTAGGGGGGTATTTCCTCGAAAGTCTCGGAGCTCAGGATCACAGCCAG10.7575757575757576No Hit
GTGTATTAACACAGGCAGGCCTCATCCCTGCACAGGTTCCAAGGAACAGC10.7575757575757576No Hit
CTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGC10.7575757575757576No Hit
TAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAGAGCC10.7575757575757576No Hit
GTACATTACAGTTTGGGAACAAAAAAAAATGTGGATAAAAAATAGTATTT10.7575757575757576No Hit
ATGCAAATCTGACCCTGAGTGAAAGAGAGAGTGATGAGGTTGCATAAAGT10.7575757575757576No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG10.7575757575757576No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)