FastQCFastQC Report
Tue 10 May 2022
EGAF00003624600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514020
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC18970.3690517878681764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16740.3256682619353332No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC13050.2538811719388351No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT12390.2410412046223882No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT11650.22664487763122057No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG10230.19901949340492586No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC10190.19824131356756544No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC9950.193572234543403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.1793704525115754No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG9080.17664682308081397No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG7450.14493599470837712No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT7260.14123964048091514No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG7180.1396832808061943No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA7070.13754328625345316No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC7050.13715419633477297No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7050.13715419633477297No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC6870.13365238706665108No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG6760.13151239251390998No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC6470.12587058869304696No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA6470.12587058869304696No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA6460.12567604373370686No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC6380.12411968405898603No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC6370.12392513909964592No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA6340.12334150422162561No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC6270.12197968950624488No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC6270.12197968950624488No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT6130.11925606007548344No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA6080.11828333527878292No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG6070.11808879031944282No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC6000.1167269756040621No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG5810.11303062137660014No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT5780.11244698649857982No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC5780.11244698649857982No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA5600.10894517723045796No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT5580.10855608731177775No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG5540.10777790747441733No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG5470.1064160927590366No Hit
GGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA5450.1060270028403564No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT5410.105248823002996No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC5320.10349791836893506No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC5200.10116337885685382No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT5170.1005797439788335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)