FastQCFastQC Report
Tue 10 May 2022
EGAF00003624621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6855
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC400.5835156819839533No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG290.42304886943836617No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT280.4084609773887673No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT280.4084609773887673No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT270.39387308533916854No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT260.37928519328956967No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT250.36469730123997085No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA230.33552151714077316No Hit
CTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCA230.33552151714077316No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA220.32093362509117435No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA200.29175784099197666No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA200.29175784099197666No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG190.27716994894237784No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT190.27716994894237784No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT190.27716994894237784No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA180.26258205689277897No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC170.24799416484318015No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC170.24799416484318015No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT170.24799416484318015No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA160.2334062727935813No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC160.2334062727935813No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG150.2188183807439825No Hit
CTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAAC150.2188183807439825No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT140.20423048869438365No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT140.20423048869438365No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC140.20423048869438365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.20423048869438365No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG130.18964259664478483No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC130.18964259664478483No Hit
CTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATGACTACCAG130.18964259664478483No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG130.18964259664478483No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA130.18964259664478483No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA120.175054704595186No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC120.175054704595186No Hit
GTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTT120.175054704595186No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA120.175054704595186No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG110.16046681254558717No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCAC110.16046681254558717No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA110.16046681254558717No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG110.16046681254558717No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG110.16046681254558717No Hit
GTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACAC110.16046681254558717No Hit
ACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCTGCTGC110.16046681254558717No Hit
GGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGG100.14587892049598833No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC100.14587892049598833No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA100.14587892049598833No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA100.14587892049598833No Hit
CTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGC100.14587892049598833No Hit
GAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGAT100.14587892049598833No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG100.14587892049598833No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC100.14587892049598833No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC100.14587892049598833No Hit
GGCTTCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGG100.14587892049598833No Hit
GAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCG90.13129102844638948No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA90.13129102844638948No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT90.13129102844638948No Hit
GCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTAC90.13129102844638948No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGTGAGCAGG90.13129102844638948No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC90.13129102844638948No Hit
GCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCA90.13129102844638948No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAA80.11670313639679065No Hit
GGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGG80.11670313639679065No Hit
GAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGG80.11670313639679065No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT80.11670313639679065No Hit
TCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAG80.11670313639679065No Hit
CGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCG80.11670313639679065No Hit
TTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCC80.11670313639679065No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT80.11670313639679065No Hit
GGCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAG80.11670313639679065No Hit
CTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGT80.11670313639679065No Hit
CTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGC80.11670313639679065No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT80.11670313639679065No Hit
ATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAG80.11670313639679065No Hit
GGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTC80.11670313639679065No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG80.11670313639679065No Hit
CTTCCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGG80.11670313639679065No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC80.11670313639679065No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT80.11670313639679065No Hit
TCTCCATCGGCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTG80.11670313639679065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.11670313639679065No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA70.10211524434719182No Hit
GTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGC70.10211524434719182No Hit
CACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCA70.10211524434719182No Hit
GCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGC70.10211524434719182No Hit
CCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTG70.10211524434719182No Hit
CTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTG70.10211524434719182No Hit
GTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCA70.10211524434719182No Hit
TTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCC70.10211524434719182No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT70.10211524434719182No Hit
GAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCC70.10211524434719182No Hit
GTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTC70.10211524434719182No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT70.10211524434719182No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC70.10211524434719182No Hit
ACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGG70.10211524434719182No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG70.10211524434719182No Hit
ATGCAGGCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCC70.10211524434719182No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA70.10211524434719182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)