FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003624625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22.564102564102564No Hit
GCAGTGCGGCGGCGCCGTCGCTGATCTGCGACGAGTTGCCGGCGGTGGTG22.564102564102564No Hit
GTTCCTGTTCGTGGCGATCCTGCCCTCGTTCATCCCGATTCCAGGCCTGG22.564102564102564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22.564102564102564No Hit
CCTGAAGGTAATCTACGACGACGGGTCTGGGGCGATGCTGGACATCCTGG22.564102564102564No Hit
GTCCCCGAAGCACAAAGCGGGCCATTGCAGGTCGGCCAAACGGCGCAGGT22.564102564102564No Hit
TGGGCGAAGGATCGTGGAGTGTGGGAAGCAGCGGAGCAGCCATTGGAAAA22.564102564102564No Hit
GATCGCCGTCCACGAGTACGGCGACCCCGAGACGGCCATGCAGCTCGCGG22.564102564102564No Hit
GTGCAGCGCCAGGTGCGGGTCGCTGGCATGTGCCGCGTCGAAATGGCCGG22.564102564102564No Hit
GACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATT22.564102564102564No Hit
CGCGATCAGCAGTGCCAGCGCGAGCAGGACCAGCACTGTCTCTTATACAC11.282051282051282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTAA11.282051282051282No Hit
GTTGAAGACCGGATCTCGGTATCGCGGACTGTCTCTTATACACATCTCCG11.282051282051282No Hit
GGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTAAATGCTGTCT11.282051282051282No Hit
AAGCGGCGGGTGACAATTCGATAGGAGATCGTCAGAAGAAGCTCGTCATA11.282051282051282No Hit
CCGCAGCCCATTCCGCGCGATTGGTCGTTGAATCAGGTAGATGAGCAGGC11.282051282051282No Hit
GGTATCAACGCAGAGTACATGGGCCGAGATCTACACTTTCTGTCTCTTAT11.282051282051282No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC11.282051282051282Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACT11.282051282051282No Hit
GGTACGCACGCACACCGATCCGAGCGCGGGGGGGGGGCCGGGCGGCCAAC11.282051282051282No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.282051282051282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCATTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC11.282051282051282No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTC11.282051282051282Clontech Universal Primer Mix Long (95% over 21bp)
GGCTGGGGCCGCCCAGGGTGCTAAGACCGCCGCGGCGGTGGCGTTATGAG11.282051282051282No Hit
ACTCTGCGTTGATACCACTACGCAGAGTACTTTTTTTTTTTTTTTTTTAG11.282051282051282Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT11.282051282051282No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAAAAATAC11.282051282051282No Hit
TCCTCGAGGCCAACCCGCAGGCCAGCCGCACCGTCCCCTTCGTCGCCAAG11.282051282051282No Hit
CTAGCACTCAAGCGACTTCTGAAGCAGATTATAAAGACCAAAGCCCAGCA11.282051282051282No Hit
GTTTTATCCCGCCGATTATTTGGTTCATGTTAAAAACGCTATTTTTCATG11.282051282051282No Hit
GTGCTAGGGCTCCGTCGTCGCGAGGGTGAGCGTCACCGTTCCTGAGTAGG11.282051282051282No Hit
AGCCTGTGCTGGTGGGCACCTCGTCCATCGAGAACTCCGAGATCATCGAC11.282051282051282No Hit
CCGCCATGAAGGCCACGTCGATGCCCTCGGGCAGCACCTGCACCGGCGGC11.282051282051282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.282051282051282No Hit
CTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTT11.282051282051282Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAATTACT11.282051282051282Clontech Universal Primer Mix Long (95% over 21bp)
GTACGGCTCGGCGGCAGCCGCCGTCACCTCGCTCACCGTGCCGACGGCAG11.282051282051282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT11.282051282051282No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.282051282051282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTTTCCTGGTTCTGCGAGCTGGACGAGCAAACGCTCCTTGCCGAGGCC11.282051282051282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTAGACGAAAA11.282051282051282No Hit
GTTGGGGTCGGCCGCGTCGCGCAGGTGCAGTTCCTCCTTCTTCGCGGCGG11.282051282051282No Hit
ATGGGAAGCAGTGGTATCAACCATGTACTCTGCGTTGATACCACTGCTTC11.282051282051282Clontech SMART CDS Primer II A (96% over 26bp)
TCGACGAGCTGCTCGAGCGCACAAGCAGCAAGTACGGCCTGGTCATCTTC11.282051282051282No Hit
AGTTGGTATGGCATCACAGTGAAATTGCGGAAGGCGAGATACAGAGGCAT11.282051282051282No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAA11.282051282051282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGAT11.282051282051282No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA11.282051282051282No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
AAGCTGGAGACCACCGGCAACAGCGTGGGGCGGCTGGACGGGCGCATCAA11.282051282051282No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTA11.282051282051282No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG11.282051282051282No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA11.282051282051282No Hit
CTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCCTCCT11.282051282051282No Hit
TTTTTTTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTC11.282051282051282No Hit
GCTCGCCACCGTTGCGCAGCGTGATGCGCACATAGGCTTGTGCGCCGCAA11.282051282051282No Hit
GCAGGAGACCCATGCCGTCGATCCTCCCAGCCGCCGGCCCCGACCCCATC11.282051282051282No Hit
TCTCGGTGGTCGCCGTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC11.282051282051282No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282Clontech Universal Primer Mix Long (95% over 21bp)
GCATTACGCACCGACACCCAACGGCTGGAAGATCTCCATCCTGCTTGAGG11.282051282051282No Hit
AATGATGGTTTCGCGAAGTTTATTGGCGACCTCAACGACCGAGTAGCCGT11.282051282051282No Hit
GTTCGTGCAGGGCCAGCGCAAAGGTCTGCACCAGCGCCTGTCTCTTATAC11.282051282051282No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGTTAAA11.282051282051282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)