FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003624626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGAAGCAGTGGTATAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33.8461538461538463No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA33.8461538461538463No Hit
CACCGCGGCTCTTCGCGCGTTGGCCCACATGGTGGTCGACCGCGACCGAT22.564102564102564No Hit
GGATGGAACTTGCCGGGTCCATCGACCACATAGGCCAGTGCGCGCTTGCC22.564102564102564No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA22.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
TGTCCACCCCGCACCGGGCGACGAGTGCTGCTCCCTCGCCCGTCGAGACG22.564102564102564No Hit
CGTCTCCGCTGCGCTCCTGCACGGGCGGCCTCAGTCCCCCGAGCCCCCCG22.564102564102564No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.564102564102564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCAACGACAGCAGGACCCCCAGCAGCACCAGCAGAAGGCCGCTGAAGGC22.564102564102564No Hit
TCGCCCCGCTGGCCGGGCTGCGCGCACGGCACGGCACCTTCGTGGTCACC22.564102564102564No Hit
CATCCAGGCGGGGGCCTACCAGCTGGCCATCGGCGGGGGGGTGGAGTCGA22.564102564102564No Hit
GTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGATA22.564102564102564No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA22.564102564102564No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAC11.282051282051282No Hit
CTTGGCGACCGGGACACCGGTGGCCTTGGCGACGAAGGGGACGGTGCGGC11.282051282051282No Hit
TAGGTGCCGTCTGCCTTGCGGGCGATCCATTCCGGGTTCCAGGTACCGGT11.282051282051282No Hit
ATAAAAGGGCACCCATGGTAAAAAACCAAAATAACATGGAACCAGGTGTA11.282051282051282No Hit
GTACATGGGAAGCAGTGGTATCAACGCTAAAAAAAAAAAAAAAAAAGTAC11.282051282051282No Hit
CACCACCAGGGTGCGGCCGGTCATCAGGCCCTTGTTGACGTCGAAGGCCA11.282051282051282No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGT11.282051282051282No Hit
CTGGCTGCCAGCCGCGAGGCCCTCCGAAAGACAGATTTGCGCCGTCGCAT11.282051282051282No Hit
GCTATGATCCGTACGAACTGGTGGCAGAGCGGTCGACGGGCGTTGGCCTT11.282051282051282No Hit
AAACATGGTCATGAAAAATAGCGTTTTTAACATGAACCAAATAATCGGCG11.282051282051282No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGCATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
GCGCTGGTGCAGACCTTTGCGCTGGCCCTGCACGAACCTGTCTCTTATAC11.282051282051282No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAATGATACCTGTCTCTTATACACATCTG11.282051282051282No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
CGATGTTGCCGCCCAGCACGATGTCGGTACCGCGGCCGGCCATGTTGGTC11.282051282051282No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGT11.282051282051282Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAACAAA11.282051282051282No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA11.282051282051282No Hit
TATCAACGCAGAGTACATGGGACGGCGACCACCGAGACTGTCTCTTATAC11.282051282051282No Hit
GCTTGCCGCCCACCCCAAGGCGGCCTACCGGGAGGCGCTGACCCGGCGCG11.282051282051282No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTACCACCC11.282051282051282No Hit
GTCCACTATGGCCGGAATGCGGTTGTTCGGGCTGATCGCCAGAAATTCCG11.282051282051282No Hit
AGCCACTGCGCTCCGGCACCTACTCAGGAACGGTGACGCTCACCCTCGCG11.282051282051282No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT11.282051282051282No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT11.282051282051282No Hit
CTCCTGCGGAGCGGTGTCGACCAGCGGGCCCACGTACTCCAGCAGGCCCT11.282051282051282No Hit
TCCGCGATACCGAGATCCGGTCTTCAACCTGTCTCTTATACACATCTGAC11.282051282051282No Hit
TAGCGGATCGGCCCAGCCGGGGCGCTCATAACGCCACCGCCGCGGCGGTC11.282051282051282No Hit
CCCCTCCTGTCATCGGGTGATTTTGAGGGTAGCCGAGATGAGGCATTGGA11.282051282051282No Hit
TCATTGGCACACGTTTGTCTACGCGTTGACCGACGGCATTGGCGACCACT11.282051282051282No Hit
CAGCGCATCGGCTGCCAGGTGCAGGCCTTCTGGCCGCCGGTGCAGGTGCT11.282051282051282No Hit
GACGGCGACCACCCAGAAAAACAAATTTTTAGTCGCAAAAAAAAAAAAAA11.282051282051282No Hit
GACCTACTTCGGCAAGCCGCTGCAGAACATCACCATCGCCGAGGCCGCGA11.282051282051282No Hit
TGGTCGACCAGCTCGCGTTCGAGCTGTGGGCGCTGCGGGCGCTCCCGCCC11.282051282051282No Hit
TATCTCGGCCGCGACGAAGATGCGAAGCGCTTCTATGATACCGATCCTGG11.282051282051282No Hit
GCGGCCAGCAACTCTCGGGCGGCGAGCAGCAGATGCTCTCGATCGGCCGC11.282051282051282No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACTGCTTC11.282051282051282No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCCTGTCT11.282051282051282No Hit
GGCTCAGAAAAATCCTGCGAAGAAAAAAACTTCTGAGGTAATAAATAGGA11.282051282051282No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGAAACCCCGGCC11.282051282051282No Hit
AAAGTGTAGATCTCGGCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT11.282051282051282No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAACTGTCTC11.282051282051282No Hit
TGCTGGTCCTGCTCGCGCTGGCACTGCTGATCGCGCTGTCTCTTATACAC11.282051282051282No Hit
GTGTTCGCCCCCAGCCATCCATCTTTCGATACGTATGGGAACATTCCGAC11.282051282051282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)