FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003624643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1099.260832625318606Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1069.005947323704333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG363.058623619371283Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG332.803738317757009Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC312.6338147833474936Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG302.548853016142736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC272.2939677145284625Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC262.2090059473237043Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG231.954120645709431Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT221.8691588785046727Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG171.4443500424808835Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA171.4443500424808835Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT131.1045029736618521Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG131.1045029736618521Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC131.1045029736618521Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC121.0195412064570943Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA121.0195412064570943Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC121.0195412064570943Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC110.9345794392523363Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA110.9345794392523363Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG100.8496176720475787Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC100.8496176720475787Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT90.7646559048428208Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC80.6796941376380629Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC80.6796941376380629Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT80.6796941376380629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA80.6796941376380629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA70.5947323704333051Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG60.5097706032285472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA60.5097706032285472Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT50.4248088360237893Clontech Universal Primer Mix Long (100% over 23bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA50.4248088360237893Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT50.4248088360237893Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40.33984706881903143Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC40.33984706881903143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC40.33984706881903143Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC40.33984706881903143Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT40.33984706881903143Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT40.33984706881903143Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.33984706881903143Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.33984706881903143Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40.33984706881903143Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30.2548853016142736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGGGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAA30.2548853016142736No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT30.2548853016142736Clontech Universal Primer Mix Long (96% over 26bp)
CAACATGCCGACCTAACTATAATTGACAACATAGAAGAAATGAATTTTCT30.2548853016142736No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA30.2548853016142736Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA30.2548853016142736No Hit
TAGACACATAGATCAATGGAACAAAATAGAGAAACCACAAGTAAAGCCAC30.2548853016142736No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC30.2548853016142736No Hit
TTATGGAATGGCAAATGAGCATTGGCTTGTAATAAGAGAGTCAGTCTGTC30.2548853016142736No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA30.2548853016142736Clontech Universal Primer Mix Long (96% over 26bp)
CAATTGTTCTTTGACTAGCCTTTTGGAGCTTTACCCATTGCATGCACAGA30.2548853016142736No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT30.2548853016142736No Hit
CAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.2548853016142736Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC30.2548853016142736Clontech SMART CDS Primer II A (96% over 26bp)
CTCAGAGAGGACCCCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAG30.2548853016142736No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG20.16992353440951571No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAG20.16992353440951571Clontech Universal Primer Mix Long (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACT20.16992353440951571Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACAT20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA20.16992353440951571Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATG20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.16992353440951571Clontech Universal Primer Mix Long (96% over 27bp)
GGCGTCTACCAGGACCCGACCTCCATCGCACAGGAAGGCGGCGCGCCGAT20.16992353440951571No Hit
GCTTATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
GCGTGCTCTGGCTGATGCTTTCGCTTAATACTTCAAAGAAGCGATTAGCA20.16992353440951571No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTGTACTCTGCGTTGATACCACTGCTTCCCTGTACTCTGCGTTGATACC20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
ACGGGGTCCAGGCCAGGCGGGGGATCCGCGTGCGCGCCCTCCGATTCTCG20.16992353440951571No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACTACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA20.16992353440951571No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAACACTGCTTCCCA20.16992353440951571Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG20.16992353440951571No Hit
CGCAAAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
GTACGTGGCTACACCACTGGCTGCCCTCTTGAACATAAAGGAGAAAACTC20.16992353440951571No Hit
GAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTA20.16992353440951571Clontech SMART CDS Primer II A (100% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.16992353440951571Clontech SMART CDS Primer II A (96% over 26bp)
ATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTT20.16992353440951571No Hit
ACCCTCGACCAGCCTGAAACCGAATCCCTAGGCCGGCGCGTGTGGTTCCT20.16992353440951571No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20.16992353440951571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGG20.16992353440951571Clontech Universal Primer Mix Long (96% over 27bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA20.16992353440951571Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)