FastQCFastQC Report
Tue 10 May 2022
EGAF00003624867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606618
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT12350.20358776033681825No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT8420.13880234348469714No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC8110.13369204342765956No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7970.13138416598254585No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7300.12033932392378728No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT7200.11869084003442035No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC7160.11803144647867356No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC7050.11621811420036991No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA6760.11143751092120577No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA6750.11127266253226906No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT6590.10863508830928197No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6580.10847023992034525No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC6550.10797569475353516No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6410.10566781730842144No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT6280.1035247882522444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)