FastQCFastQC Report
Tue 10 May 2022
EGAF00003624885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409561
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA12410.30300736642404913No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG12030.29372913924909844No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA10340.25246544470787013No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT9980.24367554527896942No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG7670.18727369061018992No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA7480.18263457702271454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.1767746440701141No Hit
CCTTTAACCTCTCCGCCCTTATCACAACACAAGAACACCTCTGATTACTC6850.16725225302213834No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT6330.15455573162483732No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG6280.15333491225971224No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6250.15260242064063717No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA5810.1418592102275363No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG5470.13355763854468566No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT5360.13087183594141044No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5320.12989518044931037No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT4880.1191519700362095No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT4740.11573367581385924No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA4440.10840875962310866No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC4390.10718794025798356No Hit
CAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGAAGTATAGT4270.1042579737816833No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC4200.10254882667050819No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT4200.10254882667050819No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA4190.10230466279748315No Hit
CATATGAAGTCACCCTAGCCATCATTCTACTATCAACATTACTAATAAGT4190.10230466279748315No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA4130.10083967955933305No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG4100.100107187940258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)