FastQCFastQC Report
Tue 10 May 2022
EGAF00003624943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461014
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9870.21409328133202027No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8510.18459309261757778No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7710.1672400404326116No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7460.16181721162480966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6770.14685020411527633No Hit
TCGCTATACGGTGCACAAGTGCACACTTTGAAACGGAACTTCCCTATAGC6460.14012589639360193No Hit
GTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGGATTGGGGTTT6410.13904133063204155No Hit
GCTAAGGACTGTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAAT5620.12190519159938744No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCT5590.12125445214245122No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5550.1203867995332029No Hit
CTTGTGCACCGTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGG5450.11821766801008213No Hit
GGGTAGAAACGATGCCCTGAGGAGTAGGCGGCCGTGGGGGTCAGTGACGA5250.11387940496384058No Hit
GTATAATACCTTTATGGGGACCGAGGTTCGCGCTTCGGCAAGGATGCTGG5230.11344557865921642No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT5220.11322866550690434No Hit
GTATTTATTGTCACTACCTCCCCGAATCGGGATTGGGTAATTTGCGCGCC5160.11192718659303189No Hit
CAATAAAGAGCATGCGGCTCCCCAGAGGGAAAGGCCCGGCCGAACCAGTG5120.11105953398378357No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT4920.10672127093754202No Hit
GTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCAAGATGT4910.10650435778522996No Hit
ATATTGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTT4810.10433522626210917No Hit
GTATTATACAATAGGCTATAACACTCCCGAGGGAGCCACATTCCTACTGC4760.10325066050054879No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG4710.1021660947389884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)