FastQCFastQC Report
Tue 10 May 2022
EGAF00003624944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461014
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9250.2006446658886715No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8280.17960409011439998No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7940.17222904293578936Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7000.1518392066184541No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6490.14077663585053815No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCT6040.13101554399649468No Hit
TCGCTATACGGTGCACAAGTGCACACTTTGAAACGGAACTTCCCTATAGC6000.1301478913872464No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5790.12559271518869275No Hit
GTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGGATTGGGGTTT5560.12060371268551498No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT5490.11908532061933044No Hit
GTATTTATTGTCACTACCTCCCCGAATCGGGATTGGGTAATTTGCGCGCC5340.11583162333464928No Hit
GTATTATACAATAGGCTATAACACTCCCGAGGGAGCCACATTCCTACTGC5210.11301175235459227No Hit
GCTAAGGACTGTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAAT5150.1117102734407198No Hit
CAATAAAGAGCATGCGGCTCCCCAGAGGGAAAGGCCCGGCCGAACCAGTG5130.11127644713609566No Hit
GTATAATACCTTTATGGGGACCGAGGTTCGCGCTTCGGCAAGGATGCTGG5010.10867348930835073No Hit
CTTGTGCACCGTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGG4860.10541979202366956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)