FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003624949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4622
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.5192557334487236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG100.21635655560363476No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT80.17308524448290782No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA80.17308524448290782No Hit
CCACTGGATTTGACTCAGAGAGGACCCCCAGAGGGTGTCTCCATCTTCCC70.15144958892254434No Hit
GTATCAACGCAGAGTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCA70.15144958892254434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT60.1298139333621809No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC60.1298139333621809No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA60.1298139333621809No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA60.1298139333621809No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT60.1298139333621809No Hit
TTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTG60.1298139333621809No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG60.1298139333621809No Hit
CTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCG60.1298139333621809No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG50.10817827780181738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACG50.10817827780181738No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT50.10817827780181738No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC50.10817827780181738No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA50.10817827780181738No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG50.10817827780181738No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50.10817827780181738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10817827780181738No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC50.10817827780181738No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC50.10817827780181738No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC50.10817827780181738No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG50.10817827780181738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)