FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003624950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4622
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA190.4110774556469061No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.3245348334054522No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT100.21635655560363476No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA100.21635655560363476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTG90.19472090004327133No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT80.17308524448290782Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.17308524448290782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15144958892254434No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCT70.15144958892254434No Hit
GTACATGGGAGTCTCGCGCCTGCAGCCCGTGCCGCCCCAGCCGCTGCCGC70.15144958892254434No Hit
GCCCCTTTCACATCAAAGAACTACTGACAACGAAGGCCGCGCCTGCCTTT60.1298139333621809No Hit
ATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTT60.1298139333621809No Hit
TCTCTACACTGGTCCAGGTCCTTCATTATTTTGTCCACAGCCAGAGGGTC60.1298139333621809No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC60.1298139333621809No Hit
TCTCTGACGTCCCCCATAGATCTTGGTCATGGAGCCAACCCCAGCGCCAC60.1298139333621809No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG60.1298139333621809No Hit
GATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCG60.1298139333621809No Hit
GTACATGGGGCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCT50.10817827780181738No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG50.10817827780181738No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT50.10817827780181738No Hit
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA50.10817827780181738No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA50.10817827780181738No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG50.10817827780181738No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC50.10817827780181738No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA50.10817827780181738Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA50.10817827780181738No Hit
CTCCAGGACTGTGGTCGCCCCGTCCGCTGTGGCGGGAAAGCGGCCCCCAG50.10817827780181738No Hit
GGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTC50.10817827780181738No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA50.10817827780181738No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG50.10817827780181738No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG50.10817827780181738No Hit
GTCTTGAAGCCCCACTTCCACAAGGACTGGCAGCGGCGCGTGGCCACGTG50.10817827780181738No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA50.10817827780181738No Hit
GTTCTAATGCTTCTTGTTGGCAGCTGCCACCTGTCCGGCGATTCTGTCCA50.10817827780181738No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT50.10817827780181738No Hit
ATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCG50.10817827780181738No Hit
GTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAA50.10817827780181738No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG50.10817827780181738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)