FastQCFastQC Report
Tue 10 May 2022
EGAF00003624954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003624954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483647
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA415518.591183239015232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT219534.539054310271748Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG159663.3011680006285578Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG139172.877511904343457Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG93301.929092912806241Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82221.7000002067623703No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT76471.5811118439688452Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC74701.544514904465447Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC71981.4882755398048575Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60761.2562881605799272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC57471.1882633408250232Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT39450.8156775499486195Clontech Universal Primer Mix Long (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG37660.77866708570507Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG36610.756957036847122Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA36400.7526150270755323Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG35610.7362807998395524Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG31030.6415836343448837Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30940.6397227730142026Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC29500.6099489917233023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG27640.571491190889223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC26910.5563975378736972Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA26300.5437850332990798No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA25400.5251764199922672Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC24890.5146315391184066Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC24300.5024325592839406Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC21720.44908786780441107Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20650.42696429420631166Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.41641941333245114No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA19970.41290445304116435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT18360.3796157114589773Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG18110.37444665220708495Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17190.35542451416012094Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC16410.33929704929421667Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA15890.3285454060502805Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA14040.29029436758627675Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA12380.2559718141537113No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11300.23364147818553616Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10740.22206278546129718Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10720.22164926072114577Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.22123573598099439No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10260.21213819169766376Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.20572855822531722No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9580.1980783505325165Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC9200.19022138046964004Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG8770.18133059855638514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT8770.18133059855638514Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT8560.1769885887847955Clontech SMART CDS Primer II A (100% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8460.17492096508403857Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8370.17306010375335731Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT8220.16995866820222189Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG7660.1583799754779829Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7510.15527853992684748No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC7430.1536244409662419Clontech Universal Primer Mix Long (96% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC7380.15259062911586344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT7340.15176357963556064Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7310.15114329252533357Clontech SMART CDS Primer II A (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG7190.1486621440844252Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG6480.1339820158090508Clontech SMART CDS Primer II A (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA6440.13315496632874804Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT6330.13088058025791538Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG6190.12798590707685564Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6170.12757238233670423No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA5550.1147531153920111Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5500.11371930354163265No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5200.10751643243936176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5190.10730967006928607No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT5180.10710290769921037Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5020.10379470977799925No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA5010.10358794740792354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4910.1015203237071666Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC4890.1011067989670152Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG4880.10090003659693951Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA4850.10027974948671242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4850.10027974948671242No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)