FastQCFastQC Report
Tue 10 May 2022
EGAF00003625063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564445
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA298375.286077474333195Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT153992.7281666061352303Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG108181.9165729167589403Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG98851.7512778038604293Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG74061.3120853227506666Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT70861.2553924651648964Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC54060.9577549628396034Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT48850.8654519040827715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC46880.8305503636315319Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40870.7240740904782574Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG30150.5341530175659276Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA28560.5059837539529981Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT26310.4661215884630035Clontech Universal Primer Mix Long (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG25480.4514168785266943Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG22550.39950748079972365Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21950.38887757000239176Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC21620.3830311190638592Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21330.3778933288451488Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20520.3635429492687507Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA18360.3252752703983559Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC17010.30135797110435913Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA16650.29498002462596No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC15860.28098397540947306Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC15650.27726350663040683Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC15510.2747831941110294Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC14990.26557060475334177Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA14660.25972415381480923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG14470.2563580153956541Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13450.23828716704018993Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT12580.2228737963840587Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12120.2147241981061042Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10940.1938187068713515Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10450.18513761305353046Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.17858250139517579No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA8730.15466520210117904Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT8180.1449211172036248Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8150.1443896216637582Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7780.13783451000540353Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7400.13110223316709332Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA7240.12826759028780485Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG6920.12259830452922783Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6830.12100381790962804Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6810.12064948754971698No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6500.1151573669710955Clontech Universal Primer Mix Long (95% over 21bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC6310.11179122855194042Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.11072823747220722No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6150.1089565856726519Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5830.10328729991407488Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG5800.1027558043742083Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC5740.1016928132944751Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5740.1016928132944751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)