FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA146.1946902654867255Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT83.5398230088495577Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC62.6548672566371683Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG52.2123893805309733Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52.2123893805309733Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52.2123893805309733Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC41.7699115044247788Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC41.7699115044247788Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATC31.3274336283185841No Hit
GGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT31.3274336283185841No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT31.3274336283185841Clontech Universal Primer Mix Long (100% over 23bp)
GCATTCAACTCATAGAGTTGAAGATTCCCTTTCATAGAGCAGGTTTGAAA31.3274336283185841No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC31.3274336283185841Clontech Universal Primer Mix Long (96% over 26bp)
GGTCACGGACTCAGAAAGACAGTTTTCCTTTGGTGTTTAATGCCTGCCTG31.3274336283185841No Hit
AGTGGCATGCTTCTTGTACAACCTGCAGAACTGTGAATCAAATAAACCTC31.3274336283185841No Hit
GTACATGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCC20.8849557522123894No Hit
GGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTC20.8849557522123894No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ACAGTGCTTAGATTACAGGAGCGAGCCACCGTGCCCACATTTTTTTTTTT20.8849557522123894No Hit
GCTCAGCGCCGAGCGCTTCGTGCAAGACCCCTTCGGCGCCCCCGGCGAGC20.8849557522123894No Hit
AATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAA20.8849557522123894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.8849557522123894No Hit
AGTTAGCTCGAAGTTCCAGTCTCTATTAAGACCTAAACATTCTTGTCCCT20.8849557522123894No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGTGTTGATA20.8849557522123894Clontech SMART CDS Primer II A (96% over 26bp)
CCATATGACCCAGCACTCCTAGGTATTTCCCGAAAGATTGAAAACTGGGG20.8849557522123894No Hit
TGGTCAATGTGTCCTGGGCCACCCAATATTGCAGCCGGGCCCAGTGCCAT20.8849557522123894No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATAATCCAGGGCTGTTGCGACAGAGACGCGGTCGTCGGCGTTCCAGA20.8849557522123894No Hit
TCACCATGTTGGTCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCTGC20.8849557522123894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.8849557522123894Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGGAGGCAGGGAAAGCACACACGATGTGGAGGCCTGGGCCGGAATT20.8849557522123894No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCA20.8849557522123894No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
CACTCAGTGAGACGCCACACACAAAAGACCACACGGTGTATGACCCCATT10.4424778761061947No Hit
GTTCTATCTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC10.4424778761061947No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATTACAGAGCCTTGTTT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCCCATGTACTCTGCGT10.4424778761061947Clontech Universal Primer Mix Long (96% over 27bp)
CCCAGATCCTGGAGCCTTACCCTGGATTAAGGGTACACCAAGGAAGCACC10.4424778761061947No Hit
TTTTTTTTTTTTTTTTTTTTTAGACGGAGTCTTGCTCTTTAGCCCAGGCT10.4424778761061947No Hit
GGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGC10.4424778761061947No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCATGGTATCAACGCAGAGT10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10.4424778761061947No Hit
GAGAATGCTTTTCTCTAGATTTTATATGTAATCCCATGAAAACTAGACAG10.4424778761061947No Hit
CTTCCTGATTCTGGAGAAAAATGCCGGTCCGGAAGCAAGATACCCAGAGA10.4424778761061947No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGTTTTAAC10.4424778761061947No Hit
CGACAGCAGCGCAGCCTTAGCCGCAGCGTCATAACCCTCCACGTCAGCCG10.4424778761061947No Hit
TCATTCCCATCCTCCACCACAGACGAAGGATGGATCAACGAGATACGGCT10.4424778761061947No Hit
CTCGTCACATGCTCACCCTATCCGTAAGCCGCCACCTCGAGAGCACACCG10.4424778761061947No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTGTTTCCAATTCT10.4424778761061947No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
CCCCAACTCCTGGGCCCCATGGTAGACTGGCCACATGGCTACTGGGCTCC10.4424778761061947No Hit
GGGAAGAGCCTGCTACGGTGGACTGTGAGACTCAGTGCACTGTCCTCCTC10.4424778761061947No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.4424778761061947Clontech SMART CDS Primer II A (96% over 26bp)
GTGTTAAAGCTCCTGTCACTAAAGTGGCTGCGTCGATTGGAAATGCTCAG10.4424778761061947No Hit
ATACGGCGTCTTGCCACCGGGCCTGTCAGTTGACCTACCCCTTGCACACC10.4424778761061947No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
CTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCAC10.4424778761061947No Hit
CTCCACATGTAGGTCAGAGCGTCCCACAAATCAGCCACGATGGATGTCGA10.4424778761061947No Hit
GGACCTTCTGTGCCCCCGCTGGTACCTGCCCGTTCCTCCAGCTACCATCT10.4424778761061947No Hit
CAGTAGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4424778761061947No Hit
ATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.4424778761061947Clontech Universal Primer Mix Long (96% over 28bp)
CGTGGATATTTTGACCACTTACAGGCCTTCGTTGGAAACGGGTTTTTTTC10.4424778761061947No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC10.4424778761061947No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAATCATTGGTAT10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGAAATTCGGGTTTTCTTTTGTCATTCTTTTGTCAAATCAAATTCGTTG10.4424778761061947No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACAAC10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GAAGACGGATGCGCGAGACGATCAGCGCGACGAGGACCAGCACGCTGGCC10.4424778761061947No Hit
ACATGGGGCGGCGGGACGCGAGCGGCGAGCCGGAGCGCGGAGCCCGGCTC10.4424778761061947No Hit
TCTTCGTGATATGCTGGCTGGAGGAGGGAGGTTGTGAGAACAAATTGCTA10.4424778761061947No Hit
ATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTA10.4424778761061947No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTATGCGTTGAT10.4424778761061947No Hit
GAGTACTTGGGAAGCATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.4424778761061947No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTAC10.4424778761061947Clontech SMART CDS Primer II A (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACAAAAGCTTC10.4424778761061947No Hit
TTCTTCTATAGCTCGCGAGCCTCTGCACTGAGATCTTCCTATTGGTGAAG10.4424778761061947No Hit
GGATTATCTTCACATTTGTATACTCAAAGAACAGAAGACACGCTGGAGTA10.4424778761061947No Hit
TCGTATGCCATCTTCTGCTTGCCCATGTACTCTGCGTTGATACCTGTCTC10.4424778761061947Illumina Single End Adapter 1 (95% over 21bp)
GTATGAGCGTGAGCGTACTGGGGTCGGTCGTACTGTCGACGTTTCAATGT10.4424778761061947No Hit
CCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGT10.4424778761061947No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
TTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAG10.4424778761061947No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA10.4424778761061947No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATCAAGCAG10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG10.4424778761061947No Hit
GTCCGCAGGCGAGGCGCGCAGCGCGCAGCCCTTCGACGCGATGGCCGGTG10.4424778761061947No Hit
CGCCGTTGCTGTTGGGGACAATCAGGCGTTCCAGCTCCGAAGAGGCGAGC10.4424778761061947No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.4424778761061947Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGT10.4424778761061947Clontech Universal Primer Mix Long (96% over 25bp)
GGCCCTGGCAACATTTGTTGAAAATATTTTTCCTCACTGTCTGCCTGGAC10.4424778761061947No Hit
CACATGTACTCTGCGTTGATACCACTGCTTCCCAGTAATCTGCGTTGATA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTGGGTGGGGCCCAGCTGTCTCCATTTTGTTTTTATTGGCTCCTAGA10.4424778761061947No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGTAATATGCG10.4424778761061947Clontech Universal Primer Mix Long (95% over 22bp)
CCTGAGATCAGGAGTTGAAGACCAGCCTCCCACAGTGCTGGGATTACAGG10.4424778761061947No Hit
GGCCCCTAAGAGGAAAAAGGAACCTCTCCTTGAGAGCGGCAGCTGATCTA10.4424778761061947No Hit
CCACATGTTTACCCCCAGTTGGAACCTCTCACTGAGTTCCAGACTCAGAT10.4424778761061947No Hit
CTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAA10.4424778761061947No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTAGCCTGGATGACTACGTGAGGTCTATATCTCGACTGGCACAGCCC10.4424778761061947No Hit
GTTTTTTCTTGGTTTAAGGACAAATGTGACAACTTCAAAGTTCCTTAGAT10.4424778761061947No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATGGGTATAA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
ATATATAACAATTTGGGGGCTTGTCTGGGATTACATTCCCCTCTGGGGGT10.4424778761061947No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.4424778761061947Clontech Universal Primer Mix Long (95% over 22bp)
CTCTTGGACTGTCTTTGCGTCGACCGAAGCCTCAGACCGCTTTTACGGCG10.4424778761061947No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTAGAAGATAAGGAAGAGAAGAAAGAAGAAGAAGAGAAAAAAAAGAG10.4424778761061947No Hit
TTCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTACTCTGCGTCTG10.4424778761061947Clontech Universal Primer Mix Long (96% over 28bp)
ATGTTTTCGTTGTGTGTGAGCAGGGAAGGGAACTTTCCTGCCTCTGTCTC10.4424778761061947No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAACAAAGCATACCAA10.4424778761061947No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4424778761061947No Hit
GTTTATCTGGGCGACACGGACCGCGCTCCCTACGGTGAGAAAACCATCGC10.4424778761061947No Hit
TAATTCTACTTAGAAAGAAATCTCGCTGACTAACCAGGGGATTAGAACAT10.4424778761061947No Hit
TGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCA10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATTAAACTGCGTTGATA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GCTATAACAAAAATACCATAAAATGTGCGGCTTAAATAACAAGCATTTAT10.4424778761061947No Hit
GGCTGCCCTGGGCGGAGGCCGAGGCGCGGCTCGGACTCCAGCATGGCGAC10.4424778761061947No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACAC10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATC10.4424778761061947Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GGATATATAGAGATTCCCACACTGCCTTGCACACGAGCACTGCTGGGTAA10.4424778761061947No Hit
GATACCACTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
ACCTAGGAGATACCATTCTGGACCTAAGTCCTGGCAAAGATTTCATGACA10.4424778761061947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4424778761061947No Hit
AGATTGGGGACGCCGATGCCATCAGCTATGACAAGGCCCGAAAGAAAGCC10.4424778761061947No Hit
TCAGAAAAGTGTTTGACAGGATCAATTCAGCAAATTAGAAATAGAAGAGA10.4424778761061947No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAAA10.4424778761061947No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTC10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGAGGATGTATGGCTGCGACGTGGGGTCGGACTGGCGCTTCCTCCG10.4424778761061947No Hit
GATTTAGACCCAGCTACATCAGTAATTAATTATAATAACTGTGAATGACT10.4424778761061947No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAA10.4424778761061947No Hit
GTACCGAGGACGGTAAAGAGCAAAAAGAGGTGCGGCGAGAGCTTATTTGC10.4424778761061947No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACACTGA10.4424778761061947No Hit
TTCCATTCCATTACAATTCGTTCCATTCCATTCTATTCCGTGCCATTCCA10.4424778761061947No Hit
GCATGCGCCAGTGCAGCGTCTGCAGGATGATCATGTCGTTGGTGGAGGTG10.4424778761061947No Hit
GCTTCTGCACGACTTTTTGCATTTATGTAAACAAGGTACGCGGGGGAATT10.4424778761061947No Hit
GTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTTGGCTGCT10.4424778761061947No Hit
TTCCTAATGTGCAGCACTGTTTTCTCTTTCAGTTAGTTGACTCAAAGGGG10.4424778761061947No Hit
GCTGAGGCACCGAGAGGAACACTACGGGAGGAGTCCAAGGACCCGGGCCC10.4424778761061947No Hit
CCACAGTACAAAATGAAGTCCGACCCTGAGGAGATCTTTAAAAACAAACA10.4424778761061947No Hit
TAATGCAGCACTCCCTCGTAATTCTGCGAGTAGGTGCGCACCCGCCCGAA10.4424778761061947No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAGTTCCGGGCTAA10.4424778761061947Clontech Universal Primer Mix Long (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)