FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125.3097345132743365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62.6548672566371683Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52.2123893805309733Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG52.2123893805309733Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52.2123893805309733Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41.7699115044247788Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41.7699115044247788Clontech Universal Primer Mix Long (96% over 26bp)
GATTTCACTTAGCATGTTTTCAAGTTTCATCCATGTTGTATGTATCAGAA31.3274336283185841No Hit
GAGTAGAGACATGGAGAGAAGGGGTCGGGGGGTGCTTGCCCCCAGAAAAG31.3274336283185841No Hit
GGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGCCCGCTCCCAAGATCCAA31.3274336283185841No Hit
GTCCACTTCCAGACACTACAAAAAGAGTGTTTCAAACGTGCTCTAAGAAA31.3274336283185841No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31.3274336283185841Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC31.3274336283185841No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31.3274336283185841Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT31.3274336283185841Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31.3274336283185841No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCA20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
CGGTAGCGACGCGGCCCTGCCCGGCCTTTTGACCATGACCATCGACCGCG20.8849557522123894No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.8849557522123894Clontech Universal Primer Mix Long (95% over 21bp)
GATTACAGGCACCTGCCACCACATCCAGCTAATTTTCGTATTTTTAGTAG20.8849557522123894No Hit
TTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTG20.8849557522123894No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ATATTACAATGTCACAACCAGGATATTAACATTGATGTAATCAAGATACA20.8849557522123894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.8849557522123894No Hit
GTAAAAGGTTTCTTTTTGGGGCAATGAAAATATTCTAAAATTGATTGTAG20.8849557522123894No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.8849557522123894Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.8849557522123894Clontech Universal Primer Mix Long (100% over 23bp)
GGTATGGATGCCCTCCTGGGCTTCCTGGGGGCTCTGCCCACTCCAGACTC20.8849557522123894No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA20.8849557522123894Clontech Universal Primer Mix Long (95% over 22bp)
CTGTAAAATTTCGCCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCGCAA20.8849557522123894No Hit
TGTGAATATTTATGCTCATACAGCACCTGACATATTTGTTCCTAAACGCC20.8849557522123894No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC20.8849557522123894Clontech Universal Primer Mix Long (96% over 25bp)
GGCAGCGCCCTGCGGTCGAGCTTGCCGTTGGAGGTGACCGGCAGCTGGGC20.8849557522123894No Hit
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC20.8849557522123894No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
TCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAAC20.8849557522123894No Hit
GTGAGTGGGCCTTCACTTTCCGTGTCTCCCCGTCCCAATACTCCGGACCC20.8849557522123894No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCC10.4424778761061947No Hit
CACCAGAGTTAGAGGAAAGAGTAGGTGCATTTTTGGCATCAGGGACATAA10.4424778761061947No Hit
ACAGTCTGTCTCCCATGTAAAGTGTGAATTACATGGGCAGTGACCAGCTC10.4424778761061947No Hit
TTCCTGCCCATGGCTACCTGTTTGTTCCTCTGCATGTTTTTCCTTTCCTC10.4424778761061947No Hit
GAGATGGACACGTCGAAGAAGGAATATCATGTCACGGATTTTACTGTGAA10.4424778761061947No Hit
CCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCG10.4424778761061947No Hit
GCCGCCTGGCAAACCGGGCAATGGTAGCTGGGAGTCGAGTCACATCAGCT10.4424778761061947No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA10.4424778761061947No Hit
GCTCCTGACAGCTGCCCTCGGCAAGGGGCACTCCGTTGTGACGGCGAATA10.4424778761061947No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAGTACACGGCGTTGAAACCAAGGCT10.4424778761061947No Hit
CAACAGAAACAAGAACTTCAGGCGCCAAGTAGGTGGGGGTTCCACATAAG10.4424778761061947No Hit
ACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTC10.4424778761061947No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTATACAC10.4424778761061947Clontech SMART CDS Primer II A (96% over 26bp)
CCAGATAAGCCGCTGATGCCCTACATGAGGTACAGCAGAAAGGTCTGGGA10.4424778761061947No Hit
GTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACAT10.4424778761061947No Hit
CATCAGAGCCGTCGCAGTGTCGCTGTCGACATCCATCGTGGCTGATTTGT10.4424778761061947No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACACTTCGTTTAT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
CAGCAGGTGGGTGCTGCCACCCACCCCCACCTGATGAGAGGGCCATCCCT10.4424778761061947No Hit
ACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGAACTG10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTGCGTTTATAACAC10.4424778761061947No Hit
GCTTCCCATTTACTCTGCGTTGATACCACTGCTTCCCATTTACTCTGCGT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
TCGTCAGACTGCGCCTGGCATTCTGCCAAGCTCCTCCCCTTGACCCGGAA10.4424778761061947No Hit
TCAGGTGACTGGGAGAAGAAATACAGCATTGTGTCTGCAGTGCACGGCTC10.4424778761061947No Hit
GTGGTCGCCCGCATTCGTGAATGCACGTCGGGACTGAAAGCCTGGCGCTC10.4424778761061947No Hit
GCTGCCCTGGGACCTGGGTGGCGGAAGCTGTGTCCGGCCCCACGTTGGGG10.4424778761061947No Hit
CCTGTCTCTACTAAAAATACAAAAATTGGTTAGGCGTGATGGTGGGTGCC10.4424778761061947No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA10.4424778761061947Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGC10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATC10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTATCAAGCAAGGACTTAAAAAACAAGTTTGAGAGAGATAGTAACAGTAT10.4424778761061947No Hit
CCGTCGCGATCACCTGACCCGGGAGGTCGGAGTTGATGCCTGTCACCAGC10.4424778761061947No Hit
ATGCACGTATCATCAGGTCTGCAAGCTGAACAGATGCTCTCACGTGTCAT10.4424778761061947No Hit
AGGCAGGAAAGTTCCCTTCCCTGCTCACACACAACGAAAACATCTGTCTC10.4424778761061947No Hit
AGCCTTGGAGGAGGCGCTGGAGCAGGATCAGAGCCTTGGGCCGGTGGAGC10.4424778761061947No Hit
GAGGGCGGTCCTGGAGTGTGCCAATCTTGTTTCGGGGAGCAGGGACTGGA10.4424778761061947No Hit
TTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGC10.4424778761061947No Hit
TGGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGC10.4424778761061947Clontech Universal Primer Mix Long (96% over 28bp)
CAAGTGGCTTTCAACATCTGCAAATGCTCGCAAACCGAACAGAAGGCCAC10.4424778761061947No Hit
TTTATACAAAGTCACAAATATAAAGTTCAGTTTGCAGAAAACACAGGGTA10.4424778761061947No Hit
CCTCCATCCTGGGGGAAGCGCCCGTGAAAACCGTGGAGAGAAGCCGCCGC10.4424778761061947No Hit
CGTTATATTTGATTGGGTTCAAATTTGGCTGATGTCCAAATGCAGCAGAG10.4424778761061947No Hit
AACTGAGTGAGGCCAGGTAGAGACTTTAGGAAGCAGGTGCGTTTTGGTGG10.4424778761061947No Hit
TGGGTAAGCAGTGGTATCAACGCAGACATGTACTCTGCGTTGATACCACT10.4424778761061947Clontech SMART CDS Primer II A (95% over 22bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGATCTCTCATA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
AGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGG10.4424778761061947No Hit
GTACAGGGGAGCAGTGGTATGAACTCAAAAAAAAAAAAAAAGACAGGAAA10.4424778761061947No Hit
GCACCACTGCACTCCAGCCTGGGTGACAGACCAAGATTCTGTCTCAAAAA10.4424778761061947No Hit
GGCTAGATGAGATCAAAAAGTTAGCCGGGCATGGTGGTGGGCGCCTGTAG10.4424778761061947No Hit
GGTCGGCCAGGCGTGCCCAAATTCGATCGTTTGCCACTGCCAAGGCAACC10.4424778761061947No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCA10.4424778761061947No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGTGAGTTGAATGCACACAACACAAGGAAATTACCGGGAATTCTTCTG10.4424778761061947No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACA10.4424778761061947No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.4424778761061947Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.4424778761061947Clontech Universal Primer Mix Long (96% over 25bp)
TGTTTCTTAAGTTTACTTCCCACTGTGGGAACAACTTGGGTTTTTCTTTA10.4424778761061947No Hit
GGTCTATAGTGTTCCAGCTTGGGCAGTTGTCATTCCACCGCCCTGTCCAC10.4424778761061947No Hit
AACCAATTCTGGACCTATCACTTCATTTGTGATTTGTGGGGAAATATGCT10.4424778761061947No Hit
CTGTTATACAGACAGCAGAGATAAGAAATAGGCAGTGGGATATCTGAGTC10.4424778761061947No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4424778761061947Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4424778761061947No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCGGTA10.4424778761061947No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTATAAAAAAGCATC10.4424778761061947No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GGGCTTCGGTGAGCATGAGGGTCGTGATTTCGTCCAAGTGCTTGGAGCCG10.4424778761061947No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGA10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAAGCAGAAGATGGCATACGACTGTCTC10.4424778761061947Illumina Single End Adapter 1 (95% over 21bp)
GCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCC10.4424778761061947No Hit
GTACTTTTTTTTTTTTTTTTTGGGTTTTTTCCCCGGGTTACCCGGGGGGG10.4424778761061947No Hit
GTACGGCAACACCACAGAGACCTGCACCATCGTGGGCCCCACCAAGAGGG10.4424778761061947No Hit
ATTAAAATCAGTGTCCTTAAATGAGAATGAACTGAATTTTGAACACTGGC10.4424778761061947No Hit
GTCATAGGCATAATCTCTCAAATTGTACCTGTTATTTTAATGATACTATG10.4424778761061947No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.4424778761061947Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTAA10.4424778761061947No Hit
GGCCCAGAGGGCGGCGGTGGCGGCAGCTACTTTTCTGGTCAGGGCTCGGA10.4424778761061947No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.4424778761061947Clontech Universal Primer Mix Long (96% over 25bp)
CCTGTCCAGGACCCCCAAGCAGCATCTCCTCCGTGGTCTCCCGAATTGTG10.4424778761061947No Hit
GCGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4424778761061947No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GATAGTGAGTGCACCCCAAGATGAGAGTGTCACAACCAGCCTCCTGGATA10.4424778761061947No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTCCACCGTGTGACGACAGAGTGAGAAGACAGCCTTCTATGAACCAG10.4424778761061947No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATACCACTG10.4424778761061947No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTC10.4424778761061947No Hit
GACAATGGATGCCCCCAAGACTATGACAGTTGGGGCCATTTCCCCATCCT10.4424778761061947No Hit
GAAACTTATTTGAAGATATTTCCTTTCTCACCATAGGCCTGAAAGCGTTT10.4424778761061947No Hit
CCACACATACTTTGTGTGGAGCTGAATTAAATTTGAATTTTAAAGGATCA10.4424778761061947No Hit
GTTCCATCCCCTGCAGGCCTGGTCTCCACGAGCTCCGTGTCCTGGGTCTG10.4424778761061947No Hit
GTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.4424778761061947Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.4424778761061947Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGGAGAGGCTGAGACTAACCCAGAAACAT10.4424778761061947No Hit
GTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGTGGGTTTTAACCA10.4424778761061947No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.4424778761061947Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATT10.4424778761061947Clontech Universal Primer Mix Long (95% over 21bp)
ACAGCGGGTCGGACGCCAGGCGTGCAGCCGTGTCCACCACGCCGCCGGGC10.4424778761061947No Hit
GATCTCCGCAGTAGTGCAGATACGCCGCACAGCGGCGCCCACGCCATCAG10.4424778761061947No Hit
GCTTCACAGAGCCTCCCTCTGGGGGCCCCCATGGGCTTGCTGCTGTCCAT10.4424778761061947No Hit
GGGTTATTCCTTGCACTGGGTAGGTCTTGATTTTTCACCCCTGAGGCCGC10.4424778761061947No Hit
GAATGGAATGGAATGGAATGGAATGGAATCAACCCGACTGCAGGGGAATG10.4424778761061947No Hit
ATTGCGCGACGGGCGGTCAAAACAGATGGATTGGCGGCAAATTGTTTTCG10.4424778761061947No Hit
CGTATATCTTCTCCCATTCTGTAGGTTGCAAATATTTTCTCCCATTCCAT10.4424778761061947No Hit
GTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTATGCGTTTATAACA10.4424778761061947No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)