FastQCFastQC Report
Tue 10 May 2022
EGAF00003625179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160699
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14460.8998189161102433Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11440.7118899308645356Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7380.45924367917659725Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6630.4125725735692195No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6280.39079272428577655No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6250.38892588006148143Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6140.38208078457239936No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA5180.3223417693949558No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG5030.3130075482734802No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4990.31051842264108676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA4760.29620595025482427No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA4450.27691522660377477No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA4180.2601136285851188No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4020.25015712605554485Clontech SMART CDS Primer II A (96% over 26bp)
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA4000.2489125632393481No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3950.24580115619885623No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT3950.24580115619885623No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3890.24206746775026602Clontech Universal Primer Mix Long (96% over 26bp)
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT3640.22651043254780676No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3580.22277674409921655Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG3540.22028761846682307No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT3420.2128202415696426No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3380.21033111593724915Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC3290.20473058326436383No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT3220.2003746134076752No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT3120.19415179932669152No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2990.1860621410214127Clontech Universal Primer Mix Long (96% over 26bp)
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG2920.18170617116472412No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA2740.17050510581895345No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG2630.16366001032987137Clontech Universal Primer Mix Long (96% over 26bp)
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT2610.16241544751367465No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG2590.1611708846974779No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT2550.15868175906508442No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA2540.15805947765698602No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC2520.1568149148407893No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC2500.15557035202459257No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG2460.15308122639219907No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG2430.15121438216790398No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT2340.14561384949501863No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA2330.14499156808692026No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC2300.14312472386262517No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT2300.14312472386262517No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG2290.14250244245452678No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT2290.14250244245452678No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA2220.1381464725978382No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2200.13690190978164143Clontech Universal Primer Mix Long (96% over 26bp)
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG2170.13503506555734635No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC2130.13254593992495287No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2110.1313013771087561Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2100.13067909570065775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2090.1300568142925594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG2090.1300568142925594No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC2080.129434532884461No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA2080.129434532884461No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT2070.12881225147636263No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG2040.12694540725206754No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG2030.12632312584396915No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA2000.12445628161967405No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA1980.12321171880347731No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1960.12196715598728057No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1920.11947803035488708No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG1910.11885574894678873No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT1890.11761118613059197No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT1890.11761118613059197No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA1880.11698890472249361No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG1850.1151220604981985No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA1850.1151220604981985No Hit
CTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATA1840.11449977909010012No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA1830.11387749768200175No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC1830.11387749768200175No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1830.11387749768200175No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG1820.11325521627390338No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG1770.11014380923341154No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC1730.10765468360101807No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1720.10703240219291968Clontech Universal Primer Mix Long (96% over 26bp)
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC1720.10703240219291968No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG1710.1064101207848213No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC1710.1064101207848213No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG1700.10578783937672295No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT1690.10516555796862458No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT1680.10454327656052619No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1680.10454327656052619Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTC1670.10392099515242784No Hit
GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG1660.10329871374432946No Hit
ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT1650.1026764323362311No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA1640.10205415092813272No Hit
GTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTC1630.10143186952003434No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG1620.10080958811193598No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1610.1001873067038376Clontech Universal Primer Mix Long (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)