FastQCFastQC Report
Tue 10 May 2022
EGAF00003625269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341972
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT9100.2661036576093949No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC9090.26581123600762635No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7090.2073269156539132Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC6370.18627256032657644No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG5990.17516053945937096No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT5840.17077421543284246No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT5720.16726515621161966No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC5610.16404851859216546No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG5400.15790766495502556No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC5380.15732282175148843No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT5330.1558607137426456No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG5270.1541061841320342No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5170.15118196811434853No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC5120.14971986010550573No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC5010.1465032224860515No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG4940.14445627127367153No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG4900.1432865848665973No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC4890.1429941632648287No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA4830.1412396336542173No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4830.1412396336542173Clontech Universal Primer Mix Long (96% over 26bp)
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA4740.13860783923830022No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA4540.1327594072029289No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC4370.12778823997286326No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA4360.12749581837109472No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG4260.12457160235340906No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC4180.12223222953926052No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA4160.12164738633572339No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT3980.1163837975038892No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC3960.11579895430035207No Hit
GTACATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAA3900.11404442468974069No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA3900.11404442468974069No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC3810.1114126302738236No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA3790.11082778707028645No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT3720.10878083585790649No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA3710.10848841425613794No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTG3700.10819599265436937No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA3690.10790357105260079No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT3650.10673388464552654No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT3630.1061490414419894No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC3620.10585661984022085No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCA3550.10380966862784087No Hit
GTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGG3510.10263998222076662No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC3500.10234756061899804No Hit
TAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG3490.10205513901722948No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG3480.10176271741546092No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC3450.1008854526101552No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT3450.1008854526101552No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA3440.10059303100838665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)