FastQCFastQC Report
Tue 10 May 2022
EGAF00003625270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341972
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC16020.4684594060332425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11890.3476892845028248No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT8820.2579158527598751No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT6850.20030879721146763No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6770.19796942439731907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC6340.18539529552127074No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC6280.18364076591065934No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC5900.17252874504345383No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG5760.16843484261869393No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC5380.15732282175148843No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG5300.1549834489373399No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT5260.15381376253026563No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG5160.15088954651257996No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC5060.1479653304948943No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG5040.1473804872913572No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4930.14416384967190296No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA4910.14357900646836583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.13480635841530886No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA4590.13422151521177172No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC4570.1336366720082346No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT4560.13334425040646603No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG4500.13158972079585463No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA4490.1312972991940861No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC4440.12983519118524325No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC4390.12837308317640042No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC4210.12310949434456622No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC4130.12077012153041769No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4120.12047769992864912Clontech Universal Primer Mix Long (96% over 26bp)
GGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA4110.12018527832688057No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA4110.12018527832688057No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTG4070.11901559191980629No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC4020.11755348391096346No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT4010.1172610623091949No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA4010.1172610623091949No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT3970.11609137590212062No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG3920.11462926789327782No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT3850.11258231668089785No Hit
GTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGG3820.11170505187559215No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT3810.1114126302738236No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC3810.1114126302738236No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC3800.11112020867205502No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA3620.10585661984022085No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT3600.1052717766366837No Hit
TAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG3530.10322482542430374No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG3520.10293240382253518No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT3440.10059303100838665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)