FastQCFastQC Report
Tue 10 May 2022
EGAF00003625401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199277
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17870.8967417213225812No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT14180.7115723339873643No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9170.46016349102003745No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA7290.3658224481500625No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7000.351269840473311No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6640.33320453439182646No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6570.32969183598709334No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA6480.3251755094667222No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6260.3141356001947039No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5690.28553219889902No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT5550.27850680208955375No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG5540.27800498803173473No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT5410.2714814052800875No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT5390.2704777771644495No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG5320.26696507875971637No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5210.2614451241237072No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5130.2574306116611551No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG4840.24287800398440362No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC4720.23685623529057542No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT4560.22882721036547116No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC4550.22832539630765217No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT4390.22029637138254793No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT4300.21578004486217678No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT4190.2102600902261676No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG4110.20624557776361546No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT4080.2047401355901584No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA4030.20223106530106333No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC4020.2017292512432443No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG3910.19620929660723513No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT3800.19068934197122597No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT3670.18416575921957878No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA3670.18416575921957878No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG3660.18366394516175977No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC3570.17914761864138862No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG3440.17262403588974143No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG3370.1691113374850083No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG3330.16710408125373225No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT3240.1625877547333611No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC3170.159075056328628No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA3150.15807142821298995No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT3150.15807142821298995No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA3090.15506054386607585No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA3060.15355510169261882No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT3030.15204965951916177No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG3010.15104603140352374No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC2990.1500424032878857No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG2910.14602789082533357No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA2820.14151156430496245No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT2760.13850067995804835No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC2690.13498798155331523No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT2610.1309734690907631No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2590.12996984097512507No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC2570.12896621285948703No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC2520.12645714257039198No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT2510.12595532851257296No Hit
GTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCAC2510.12595532851257296No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG2470.12394807228129688No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA2470.12394807228129688No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC2420.12143900199220181No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG2420.12143900199220181No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG2400.12043537387656379No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG2400.12043537387656379No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA2400.12043537387656379No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT2380.11943174576092574No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA2380.11943174576092574No Hit
GTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCC2380.11943174576092574No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC2380.11943174576092574No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC2370.11892993170310674No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA2350.1179263035874687No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA2340.11742448952964968No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC2310.11591904735619263No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG2280.11441360518273558No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA2280.11441360518273558No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC2270.11391179112491658No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT2260.11340997706709756No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA2250.11290816300927853No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA2250.11290816300927853No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG2240.11240634895145954No Hit
ATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCA2230.11190453489364051No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT2230.11190453489364051No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG2210.11090090677800249No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG2210.11090090677800249No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG2200.11039909272018346No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA2180.10939546460454544No Hit
CTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTG2130.10688639431545034No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA2120.10638458025763134No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC2110.10588276619981232No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT2100.1053809521419933No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA2090.1048791380841743No Hit
TCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA2090.1048791380841743No Hit
GTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCA2080.10437732402635527No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA2050.10287188185289824No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC2050.10287188185289824No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC2030.10186825373726019No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC2020.10136643967944119No Hit
CCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACA2020.10136643967944119No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT2020.10136643967944119No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA2000.10036281156380314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)