FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCAGTGGAAAGACCACATCATCAATCTCATCGATACCCCGGGCCACG38.333333333333332No Hit
GTGTTCCAGCGACCGACGCCCTTCCCGACGATGAACATCCGCGACAACGT25.555555555555555No Hit
GAACACGGCCACGCGCGGCTGCACGCAGGAAAACAGCTTTAGCACCACGG25.555555555555555No Hit
CCCCTAGAGCGTCTCCAAAGCGCCTAGCTTTTGCTTCGCGCGCGCACGCG25.555555555555555No Hit
CCGTCGGGCCGCGCCCGGGTCGGGGTCAGCATCGGCGTCGCCTTCTACCC25.555555555555555No Hit
ATGCTCAGGCGCAGGCAGGAAGGGATGCGGCGGATGTCGTCGCGGAACAG25.555555555555555No Hit
GCCGCAGCGCGGTCACGTCGCCCACCGCGTTCACGTCCTCGCCGTCGATC25.555555555555555No Hit
AAAGAGAAGAAAGTATTCCCGCACCCAAGTTTAAAGAAGATAAGAAAGTT25.555555555555555No Hit
TTATGGAACAAACAAAAGAATGAAAAGCTAGCATCTTGTTGCGATGAAAC12.7777777777777777No Hit
TAGCTGAGCATGACTTGTTCTGGGTGAATCTGTGCTAGTTTCTAGTGCCC12.7777777777777777No Hit
GTTGCCATGTAAGCCACACAAACCGGCATCATTGTCTTGTTTTTTACGAT12.7777777777777777No Hit
TGCCGGCTCTTGGCGATGGTCGAGAGCGTGACGGGATCCGCAGGCGCCTC12.7777777777777777No Hit
CGCCGAGCCCGATGCAGACGCGCAGTTCGTGAAGCTCGCAGACGAGGCAT12.7777777777777777No Hit
GCCAGAAACGGCGCCAGGCGCCGGCGCCGTCCATCGCGGCGGCCTCGAGC12.7777777777777777No Hit
GTGCTGGTGAAGGGGCTCGACCGCTTCGGATGCGAGTTCATTCAGGTGTA12.7777777777777777No Hit
CTGTTACCGGATCGCCATTCGACCATTTCGCGTTATCGCGCAGATGGAAA12.7777777777777777No Hit
CTGCACCTCGTGCCGCTCCTGGACCGGGGCCTGAGCGCGCGCCTCGCGAC12.7777777777777777No Hit
GGCTACGACATGATGGGGCGGCTCCCGGTCATTCCCGGAAAGTCACTGTG12.7777777777777777No Hit
GCATTGGCCGCCGCACAGCCGGATACGCCGCCACCTATCACCAAGACCTC12.7777777777777777No Hit
GTCCACAGCTGAGCGGACGGGCACGTTGGCAAGTTATCCACAGGTACAAA12.7777777777777777No Hit
AAGCAAATGAATGCTATTAAGAATCACGATTTATGGGAAGCATTGAAGTG12.7777777777777777No Hit
TCATTCCGCTGGCCTTCGGCCTGCGCGAGCCGGGCTTCCAGCCCGGCAAC12.7777777777777777No Hit
ACAGTCGGCCGAGGAAGCCCACGGTCACCGGCTCATCGGCATCGCGGGCC12.7777777777777777No Hit
ACAGAAAACTAGTCAATAAGAATTATATATCCTGCCTAGATTACCTGATG12.7777777777777777No Hit
TACAGACGTCTAAATATTTGATTCTGCATTCATCTTTTTTTTTTTTGAGA12.7777777777777777No Hit
TGGCCACACTGTTATTTTTGAAAGGAATGACATTTTTCCCTCACTCCAAA12.7777777777777777No Hit
AATCAAAACATACACACCCCCACAAAATCTATAAAAACAATTTTATTTCT12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)