FastQCFastQC Report
Tue 10 May 2022
EGAF00003625463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445107
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT12910.29004261896577677No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA12060.27094608712062496No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT8790.19748060578692314No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT8790.19748060578692314No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT8770.19703127562586076No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC8560.19231330893470558No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG8240.18512402635770725No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC7650.17186878660636656No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT7530.16917280563999218No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC7140.16041086749927547No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT7110.15973687225768185No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC7100.15951220717715064No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC7010.15749022145236988No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC6990.1570408912913075No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA6890.15479424048599552No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC6870.1543449103249331No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA6720.15097493411696514No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC6710.15075026903643396No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC6560.14738029282846596No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG6380.1433363213789044No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT6340.1424376610567796No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC6340.1424376610567796No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT6300.1415390007346548No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC6280.14108967057359242No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT6260.14064034041253No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6260.14064034041253No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT6250.1404156753319988No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA6120.13749502928509325No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA5990.13457438323818766No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT5970.13412505307712527No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG5790.1300810816275637No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC5740.12895775622490774No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG5690.1278344308222517No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT5680.12760976574172053No Hit
CCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCC5640.12671110541959574No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5630.12648644033906453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG5460.12266713397003418No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5350.120195818084191No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC5280.11862316252047261No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG5150.11570251647356702No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC5150.11570251647356702No Hit
ATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTC5130.11525318631250463No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC5010.11255720534613024No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA4980.11188321010453667No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC4970.11165854502400546No Hit
GAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGG4900.11008588946028709No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG4890.10986122437975589No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC4870.10941189421869349No Hit
GTGTAGTAAGGGTGGAAGGTGATTTTATCGGAATGGGAGGTGATTCCTAG4790.1076145735744439No Hit
GTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTC4720.1060419180107255No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA4720.1060419180107255No Hit
ACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAG4700.10559258784966312No Hit
GCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA4670.10491859260806952No Hit
GTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC4520.10154861640010156No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC4500.10109928623903916No Hit
CTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTAT4480.10064995607797676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)