FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003625531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCATGTTCGCGGAGTTCGACGAGGTGAAGGGCATCGCCGACATCTTCT421.052631578947366No Hit
CACCACGCCAGCCAGCTGGCCGAGGGCGCCGCGGCGGTCGCCCGCCAGGG315.789473684210526No Hit
CGGATGGACGATCGCTTTCATCCTCTACAAACCCGCCGCGGCAATCGTGT315.789473684210526No Hit
ACACCCAAAAAAAAATAGAAAAAAAAAAAAATAGAAAAAAAAAAAATCAA210.526315789473683No Hit
GCACTAAGACTTTGTTCGCTTGGATGCGCTGCCCCTTGCTGTCTCTTATA210.526315789473683No Hit
GCCATGCGCATTGTCAATGACAGTGAACAGTTGATGAATTACATGGATAA15.263157894736842No Hit
AGTGAGATGAACTGTAAGTGCTGTGTCAAGCTCCTCCTGTCTCTTATACA15.263157894736842No Hit
TTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTT15.263157894736842No Hit
ACGTACGTCCGCGACGTCGACGGCCGCGCCGCTAAATGGTGTATCGAGTG15.263157894736842No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACACGCTGATCTCGTA15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)