FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCAAAAATTCACAGCAAAATCTTACTTAAAGGTCTGATCTAATAAGC33.4090909090909087No Hit
CAATGATAGACTGGATAAGGAAAATGTGGTACATATACACCATGGAATAC22.272727272727273No Hit
GAGCTATGGTTGTGCCACCGCACTCCAGCCTGTTTGATAGAGCAAGACCC22.272727272727273No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22.272727272727273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCGGCGCCGTGATCGCGCTGACGCGCCCGCAGGCCTGGGCCACGGTGC22.272727272727273No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGTACAGTGAA22.272727272727273No Hit
ATTGCATTCGAGTCCATGGATTATTCCATTCCATTCCATTAGATGATTCC22.272727272727273No Hit
CCCCGTTGCTTTTCAGCATCCCTATTTCTAGTCCCCGTTTGCTAGTTAGT22.272727272727273No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGC22.272727272727273No Hit
AAACCCGAGTGGAATGGCATGGAGTGGAGTGGAATGGAATGGAATGGAAT22.272727272727273No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC22.272727272727273No Hit
GTTACGGAATCTACCATTTTAAAGTTAATTGCTTGTCAAGCTATAACCAC22.272727272727273No Hit
TTCGTGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGT22.272727272727273No Hit
TTCTTTCTCTCTCTCTCTCTTTCTTTCTTTCTTTTCTTTCCTTCTTTCCT22.272727272727273No Hit
GAATGTAGGTGTCTTGGGAAGTTGGATAGTCAAGTATTTCTAGAGGGTTA11.1363636363636365No Hit
GTGCGCCGTCATGGGTGGCGAGGAGGTGCTTGCCCGTGTCGAGAAGAAGC11.1363636363636365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1363636363636365No Hit
CCAGGCTAATTTTTTGTATTTTCAGTAGAGACGGGGTTTCACTGTGTTAG11.1363636363636365No Hit
GATGAAACCCAGACACATAGCAATTCAGGAAATTTGACTTTCCATTCTCT11.1363636363636365No Hit
CTTCAGTACCCCAACTTCAATAATGGATGCAACAATAGACAGAAGCTCAA11.1363636363636365No Hit
GGCTGGAGTGCAATGGTGTGATCTCGGCTCACTGCAACCTCCGCCACCCG11.1363636363636365No Hit
AAGTAAGTAGCATTAACGTGCTTAAGATTCTACAAACACTTTTTCCTTAG11.1363636363636365No Hit
GTCTCCAACGCTCGGCGGCCCCGAACCGGCCGGTGATGCCAAGCTGCTTC11.1363636363636365No Hit
GTGATAGGGAAAGCACACACACACACACACACACACTCTCACACAGTGTC11.1363636363636365No Hit
GCCCTTCTTGACGCCACCTACCTCACCCGAGATGTCAGTTGCCGCCGGGA11.1363636363636365No Hit
CAATCAAATTAGAACTCAGGATTAAGAAACTCACTCAAAACCACACAACT11.1363636363636365No Hit
GGAGAAAGAGAGGGAAAGAGAGAGAGAGAGAGAGACAAAAAAAAAGAGAG11.1363636363636365No Hit
ATCTTAGATATATCTGGTCCATGGGGTGCAATATTAAACATATTCAGTGC11.1363636363636365No Hit
CTTTTCAGACAGCTATGAGTTTGAATTTTGTTCCACCATTTACCAGCTTC11.1363636363636365No Hit
CGATTATTCCATACGATTGCATTCGATAATTCCATTCGATTCCATTTGAG11.1363636363636365No Hit
ATTTAACTGTTGTCTCAGAAAGAGTAAAAATTTTAAGCTCTTTGACCTGT11.1363636363636365No Hit
CATTTTGGGTATAAGTCTGAGATGCCCATTAAAAGTCCTGGAAGCAATGT11.1363636363636365No Hit
CATAGGATCTGATGGTTTGTTGTTTGAATAACCAAATCATTGTTTTTGCA11.1363636363636365No Hit
GTATATGGGAAGAAGGAAATAGGAAGCAGTTGTCAAAATAATACTGACAC11.1363636363636365No Hit
ATCCTGTTACAATTGTATTCTTTATATTTTAGCTGGTTAAGCTGAACAAG11.1363636363636365No Hit
GTTTTATCTAAGGAGACGGAATTTTCCAATACTGTAGAGGCATGTGTGTG11.1363636363636365No Hit
GTACAGAGAGAACACGGTATGAAGACACAGGGGGAAGGAAGCCATCTATA11.1363636363636365No Hit
ACCTAGAGCTTGACTTATAGAAGGTACTAAAACTAAAGGGAGTTGTTGTT11.1363636363636365No Hit
CCAGAAGGATATTCAAAATTCCCAGGATATATCAAAGAGTTCAAGCTAGA11.1363636363636365No Hit
GCCCAGAGAGACCGAACCCCATAACAATATGCACTGCCGATGGAGAGCCA11.1363636363636365No Hit
ATGTAACAGATCTGCACATGAACCCCTTGAACCTAAAATAAAAGTTGAAA11.1363636363636365No Hit
ACGAATGTCTGAATCAGGCTTTAAACTGTTGTGCCAGTGCTTAGGCTTTG11.1363636363636365No Hit
GTGTGCCTGTCCAAAACAGGTAATTACCTTGGCCAGAGTTTGCTATATGA11.1363636363636365No Hit
AGCAGTGACCGCCGCGACCATCTCCCCGGCCCGCGCCTGGATGTTAGCCG11.1363636363636365No Hit
ACCAAGACATCTAATGCCTATGCTATTCCGGGCATTGTGCTGGGCACCTT11.1363636363636365No Hit
ATGTAAAGGTCTTTATATATCCTACATCTGCAACATTGTTTTTATTTTTT11.1363636363636365No Hit
GTTTGATTTATACCTAGGTAGTTTTTTTTTCCCCCTCCTTTTGGAGCTAC11.1363636363636365No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT11.1363636363636365No Hit
GCCCTGAAGCCGGTGCGCAGGCACATGGTGAGCCCGAAGATCGCGGGCGC11.1363636363636365No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
CTTAGCAACTGGTCACTCATCCTCTGGCAGCTGGATCTTGCTGGGGTCGA11.1363636363636365No Hit
AACTGTAGTTACTTCTTTCACAGCAGGGAAGGAAGAGGGAAGAGGCAGCT11.1363636363636365No Hit
GGAAAGGACTTTACAAAAAAAGAAAACTAATTACCAATACCCTTGATGAA11.1363636363636365No Hit
CAACTAATACAAACGCCCAGCTTAAGACTAGAGGCACACGCATTTCGCAC11.1363636363636365No Hit
GTGGGATGGAGTGGCTGGGCCGTTTCTCAGTCTTGTGGATCATTACATAA11.1363636363636365No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
CACTTGTCTGATGAAAATTGACAAAAAAAGTGGAAAAGTGCTTCACACCG11.1363636363636365No Hit
CTGCCGTTGAGCGAGAAATGAATAAGACGGTTTTGCCGATGATGCACGGT11.1363636363636365No Hit
GGTTTTGGGGGTGGATTTGTGTGTCTGTGCAGGTATGTTTGTACTTGTGG11.1363636363636365No Hit
GTGTACGTGACCCGGCGCTGCACGGTGGTGTCGGTGCCCGACTCGCTGCT11.1363636363636365No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTC11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
CTACAGGAAGCGTCCCGTCGCCGTCGGGCTCAGTGCCAGGAGCCGGCGCT11.1363636363636365No Hit
CGTGTGAACTGCCATGTTGATTTCGTGCTGTCTTTCAGAATCACTATCAG11.1363636363636365No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
CATTAGGAAAAATGGAGAAGGTTTACTCTTTCTTCCATAATTTCTCCTGC11.1363636363636365No Hit
CCATTACTCTAATTAACTTTCAGTATTTTTTTAAGCTCCTTTGATTCCCC11.1363636363636365No Hit
GATCTACCTGACTGTTTTTGGCCCATACCACTGGCCTCCTCCCACTGTCC11.1363636363636365No Hit
GAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTAC11.1363636363636365No Hit
TCACTGATAAATGCAAGTAAACAAGCTGGAATTTTTCTAAAGTGTAACAG11.1363636363636365No Hit
AACTAAAAGTGTGTAAAATTTATATGGCATATCATCATCATCATCAGCCT11.1363636363636365No Hit
CGTTTAGTTTGATGGCTTCCTGCCTCCAAATTGGCAACAGTGTACCTCAC11.1363636363636365No Hit
CATTAACATTTAAGTCTAGAAGTGATAATATCTCTGGGAAAAAAACTTTT11.1363636363636365No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA11.1363636363636365No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)