FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGAGATCAGCCTGTATCAATAGTGATACATCTCAATTACCAAAACAC33.4090909090909087No Hit
CATTTATTTCGGCTTCTTTTATTCCAGGATTAATGTAGTGTAACATTTTC22.272727272727273No Hit
GCCAGATTCACTTGTATACCAAATTATCCCACTAATTCTCTTTTCCAACC22.272727272727273No Hit
CCCGCCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGAT22.272727272727273No Hit
CACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACT22.272727272727273No Hit
TCACAGAAGCACCTCTCTCCCTCGCTCCGCGTCTTGGCGGCAGTTGGTGG22.272727272727273No Hit
CACCTGTAGTCCCAGCCACTCGGGAGGCCGAGGCCGGGGAATTGCTTGAA22.272727272727273No Hit
GCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCC22.272727272727273No Hit
CCATTCCAATCGAGTTGATTCCATTCCATTCCATTCCATTCCATTCCATT22.272727272727273No Hit
CTCACCGAGCAGCCACCAGGCCAGGCCGACCGCCCACAAGGGCATGAGGT22.272727272727273No Hit
GACTCGAATGCAATCATCTTCATATAGAATCGAATGGAATCACCGAATGG22.272727272727273No Hit
TTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTTCTATCAT22.272727272727273No Hit
GGTTTGCTGCACCTATCAACCCATCACCTAGGTATTAAGCCCAGCATGCA22.272727272727273No Hit
NGTGGGTTCATGCAGACATTCAGGAATGGCTTAACATATGAGAGTCAATA11.1363636363636365No Hit
GTGTAGGTGCTCTGGGAGCCCTGGGCGCCGGGTGGCTTCATCGCCTGCAC11.1363636363636365No Hit
ACTTAATGGATTGAAGTGATAGAAGAAGAGAGAAATATGGAGTTTTCTAC11.1363636363636365No Hit
CTCTGAATCAGGCTTTCAAAAATATCGGTGTGAAGCACTTTTCCACTTTT11.1363636363636365No Hit
CGGCGATCAGTCCGCTTTGCGTGACGCGTGTCGGGCCGAGTCGTTCGTGG11.1363636363636365No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGA11.1363636363636365No Hit
GAACATGCGACTTGTCTTCTTCTTTGGCGTCTCCATCATCCTGGTCCTTG11.1363636363636365No Hit
GAGCATATCTTCCCAAATGAATAAGTTATGGGAATACCACAATATTGATC11.1363636363636365No Hit
CAGTAAATGCGTTAAATTGTATCTAAAGACAGAAAGTAAAATATGAAGAG11.1363636363636365No Hit
GATCAGAATACACTGTATGTTTTATTGTGATTCAGCTCTTCCTTCCACAT11.1363636363636365No Hit
GCATACAGAGAGCTGCCCTTTCCTGTCTCCCCAGCCCTTCTCTCAGCCTG11.1363636363636365No Hit
GGAAAATGTTCCATGTGTGCTTGACAAGAATGTGTATTCTGCTGTTGTCA11.1363636363636365No Hit
GCAGGAACCTGTTTCTCAAAAGGCTGCAGAAAGTTAGCTGTGATAGGATA11.1363636363636365No Hit
ATCCTGGCTAACACAGTGAAACCCCGTCTCTACTGAAAATACAAAAAATT11.1363636363636365No Hit
GGAGGCAAAGACCTGCATGAAATAGAGAAGACCTGCCGTGCAACTGGCAA11.1363636363636365No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
AAGCACTACATCATGCTAAGAAGAACAGTTATTACTCAGCTGCCATACTT11.1363636363636365No Hit
CCTTTGTGCACGAGCCCCTGGTAGATGTCCAGAATGTCTGTTTCCAGGAA11.1363636363636365No Hit
CATCTTAGTTGGGATTAACCACATTTCAAATGCTCAATCCACACACATGG11.1363636363636365No Hit
CCTTCTGTGGCCGAATGTGCCAGAGCTGTGTTTGCTTCCTTCCTCTGGCA11.1363636363636365No Hit
GTACATGGGGACGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGG11.1363636363636365Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA11.1363636363636365No Hit
GAATATAGGGCAGCAACTCATAATCATACACACTAATGTTTCTGTAGCAA11.1363636363636365No Hit
GCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAA11.1363636363636365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTGCGTTGATACCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1363636363636365No Hit
CTTGGCAACTGAGTCACAGATATCGTGACTTTAAATCTTTAGGTTAATAG11.1363636363636365No Hit
CTCCAGGCTCAGCCATGGAGGCCTTGGGCAGTGGCACTGAGTCCCGAGGC11.1363636363636365No Hit
CTCCCAAGGCAGTCCAATCTTCTTGTTTTTCAATATAATGTCCCTCAAAG11.1363636363636365No Hit
CTTCAAGGATTTCACTTGTTATCATGTTATTTGCACCCTTAACGAAGCTT11.1363636363636365No Hit
TGGTAGCCCAGGCCGTCGAGCAGCACCTGCGCCATGGCGCTGGTGGCGAT11.1363636363636365No Hit
ATACCCAAGGCTGCAAATCTGTTATTCAGTACCTGCTACACTTTTGGGGT11.1363636363636365No Hit
CACCCTGATCGGTCTCGCCCGGCAACTGGCAGCCGGGCGCCTCGACCTCA11.1363636363636365No Hit
GCCGGAAGTAGGGCTAAAACCGTGACCGTCAACCATTAATAAGGGTAACC11.1363636363636365No Hit
GCTCATCTAGGGAATAAATGAGGAATTGAACTTAGTTCTCTACACCTTCA11.1363636363636365No Hit
CTCCCTGACGGTCCCACTTCTCTCCAATCTTGTAGTTCACACCATTGTCA11.1363636363636365No Hit
GCTGCATGTAGCCTGAGCTGTCACTTATGACTATGGCTCTCTCTGTCCCT11.1363636363636365No Hit
TAGTTGCAAGGCATTGGTGTATAATATAAACTGATGATTTGGCAAAAAAA11.1363636363636365No Hit
GTTACTGGGATGATAAACACATATGCTGTCCATTGAGAGTGATTACTATG11.1363636363636365No Hit
GTAAACTTCCCTCTACACTTTGCTTTAAATGTGTCCTGGAGATTCTGGTA11.1363636363636365No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACAGGTTAACTTTTTAAAACATCACTTTTATTTCTATACAAAGACA11.1363636363636365No Hit
GGTAAGATCTGAGTGTAAACTTTACATACATGAAGTATGAATGGAAGGTA11.1363636363636365No Hit
TCATTAAGATGGTTTTGGGTATTCAGGAACAAGCATACTATTTCACATTA11.1363636363636365No Hit
GGTCAGGCTGGTCTTGAACTCCTGACCTTGTGACTTGCCCGCCTCAGCCT11.1363636363636365No Hit
GGGTAAGTCCCCGTCGAAGGATGAAGTGATCTCGGCGTTCGTTGAATGGG11.1363636363636365No Hit
GGAGCAGAGAGTCTGGGCTGAAGGCCAGTGCTCTCAGCAGTGAAGTTACA11.1363636363636365No Hit
CCGAGTAGCCAAGCGGCAGCAGCTCGTCACCCAGCGAGCCCTCCTTGTGC11.1363636363636365No Hit
GAAACACTTGTGCCTGGGTCGTGACTGCCCTCAGCATTCCTTCTGGGTAG11.1363636363636365No Hit
TCTCATATCAGTGCAGTAATTTTCTTGTAAAATCATTCTGTCATGAAATT11.1363636363636365No Hit
GAATATAGTCATACTGCTTGTTGAGGAGTTCTTATGACCCAGGCCCTGGG11.1363636363636365No Hit
ACCTTATTCACCCTCAAAGTTATTGGCTTTACAACGGTTGGGGTCAGAGG11.1363636363636365No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
TAATCACTTTATTCAAATTGTTTAAATATTTGCTGGGTCATTGTTCATAG11.1363636363636365No Hit
ATATTCGGATCTGGAGCCTTCCAGCCGTTCTGCTTAGCTAGCCGGACACC11.1363636363636365No Hit
GACTATTATCAACATAATCTGCCCTTTGTCTCGGAAAATCACACTCTCAG11.1363636363636365No Hit
CTCATCATCGAATGGATTCGAATGGAATCATCGAATGAAATTGATTGAAA11.1363636363636365No Hit
GAATCGCTTAAACCCGGGTGGCGGAGGTTGCAGTGAGCCGAGATCACACC11.1363636363636365No Hit
GCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTA11.1363636363636365No Hit
GCGCTGGGCTACTACCTGCTGCCGCAGCTGGCGGCCAACTGGAAGTCGGC11.1363636363636365No Hit
CCCCCGTTAGGAACGCACGCCGGACATTCTGTTTTGCCCAGCAGTGAAGT11.1363636363636365No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)