FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length100
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGGAAAGCCAAGCGCTGCATCAAGGCCCCACTCAGCATGTCCTTCCG35.084745762711865No Hit
ATGGTTAGCCGTGCTTTCATCAATACCTTTTTGTCCAGCACCCTCAAGGA35.084745762711865No Hit
ACTCTGGCGAGTTCAACGGTCTTGACCATGAAGCGGCCTTCAACGCCATC23.389830508474576No Hit
CCCCGCCTGCTGGAACTGTCCGGCCTCCGCCGCATAGGTAACTACGCGGG23.389830508474576No Hit
GTATTCCGAGGCCAAGTAGCACCAATGCCGACGTCAGAGAAAACAACAAC23.389830508474576No Hit
AGTACCGGCTACGTCGCCGCCGCGCTCGCACTGCACGGCAGCGGTCCGTT23.389830508474576No Hit
GCTCGCCCCGCTGTTCCGCACCGACTTCACGCGCCGCCTGTGGCACGTGG23.389830508474576No Hit
GAGCGGGGCGTGTCATCGTTCCCCGACTCCGTTTCCTTCGCCATTGACGA23.389830508474576No Hit
TTCCAGGAATTCCGTCAGCGTGGTGAGATCAACCACCGCCGCAGCATCGT23.389830508474576No Hit
CATGAGAATCGGTTCCGTTATACTCCGCCGCATTCTAACAAACCGTCTTT23.389830508474576No Hit
GACCTCGGATGTGTCGGTCGTCGACCTGACCGTCGAGCTGGAAAGCGCCG23.389830508474576No Hit
GACGAGCAAACCCACAATTTTACCGAGCGCCCTGCAGACCCATGTCGCCA23.389830508474576No Hit
AGTTTGCGCGCGGTGCCGTGATCGGCCTGATGTTGCTGCTGCCGGCGCTG23.389830508474576No Hit
GGCTGCCCTGGGCTGAGCGCCGGTCCGCGCACGCCACCCCCCGGAGCCTC23.389830508474576No Hit
CCCTCGAACCGCTCGCCGGATTCAGGCAGTTCAGCCGAGACGCGCGGGCT23.389830508474576No Hit
GAGTAACTGGAATCGGGCTAACAACAGTTGAACATGCACGAGCAGAGTAC11.694915254237288No Hit
TGTCGGTCCAGTAGGCCGGCACGACGCCAGATCATATGGCGGAATTGTCG11.694915254237288No Hit
GTATCGGCCTGGACGCTGTCGAAGGTGACGACCGCCCCGCGCGAGACGCC11.694915254237288No Hit
GGGTGGCGTAGGCGCGAAACGCGGCCGGATTGGCGTTCTTGCCCATGCCC11.694915254237288No Hit
TTTTGTGGGAGGCGGTTTTTGTTTTTGTGTTGGTGGTCTGCCTCCCTTTC11.694915254237288No Hit
CCTCCAGCCCCTGCTCACGCGTCGCAAAGCCGAACTCCTCAATGCCAACG11.694915254237288No Hit
TGTGCCACAACACCAGCGACATGACAATGGCGATGAGCGAACCGCCGATC11.694915254237288No Hit
CACTAGAGGTTGAGCCCGAACGATAGTCTCGGCTTGTTGATCTTGCAAAG11.694915254237288No Hit
TTTGCCGTGTGTGCGTTGATGGTGGCGCTGGCCAAGACGCTGGGGCTGCC11.694915254237288No Hit
GCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATA11.694915254237288No Hit
TGTGTACAGTGGGGCCCCCTCCATGTTGCCCCAGCTGGCCTCGAACTCCC11.694915254237288No Hit
ACCCGAGCCAGCCCGGCAGGCTCTGGGTCGGCACCCGCTACGGGCTGAAC11.694915254237288No Hit
ACTCCCGCAGCTTGCCCAGGCCAATGGCGCCGAGCGCCCGTTCGTAGAAC11.694915254237288No Hit
GTTGTTGGCTGGGCTGGCTGCTGCGCAGGACCCCTCCAAGGTCTAGAGGG11.694915254237288No Hit
GGAGCATGGCTTCGAGCCGGCGCAGCATGCGGTCGGTGCGCCAATCCTTG11.694915254237288No Hit
CCCCAGCGGAACGGTGCATGGAACGGACCCCACAGCCGGTCGCCGTTTGG11.694915254237288No Hit
GAATACTGCGTATTCGGCTGCAGCGGCGACGCAGGGTATGCGACGGCCTG11.694915254237288No Hit
CCGCCAGCCGGGTGGTGGCGGCCAACTGGTCCCAATCCAGACGACGCTTG11.694915254237288No Hit
CTACCAAACCAGTCAGCAGGGCTTGGACGGAAACCGAACGCTAGCAAAAC11.694915254237288No Hit
ACTCGGGCGGGTTCGACAAACGCGGACGGCGGAACTGATGACCGTACGCA11.694915254237288No Hit
GCCATGCATTGGCGCAACTGCTCCAGCCCGCGCCGTATCCAGGTCTTGAC11.694915254237288No Hit
GTCCAGGCCAATGTCCTGCATGGCGAGCGCCATGCAGCGCATCCATTGAT11.694915254237288No Hit
GTCCATGATGCGCGGCGATAGCGCGCCCATGCCGCCGACCCGCCCTGCCT11.694915254237288No Hit
CTGCTGGAAAACCAGGCCAGCAGCGTCTGGTCGATGGGCAAGGATGGCCG11.694915254237288No Hit
GAAGTGGCCTCTGCGCAAGAGGCTCGTGAGATCTGTTGGATGAAAGCAGC11.694915254237288No Hit
GGGTCGCGAGGCCCTCCAGGACTGGTCGCTCCGACTCGCTTCCGAGAGAC11.694915254237288No Hit
TCTTCAGCGTGTAGCGGCCCTTCGGCAGGCCCTTGAGCGAGACGGGCGCG11.694915254237288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)