FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATCGCTACAGTAAATGTTTCGATAATGGCTTCCTGCGGGAAACCT33.614457831325301No Hit
CGACGAACGCCCCCGTCGCGCCGGGGTCAGCTCCCTGGGCGTGGGCGGCA33.614457831325301No Hit
TCACAGATTTGCTCGGCAATCAGTACTGGTAGGCGTTAGACCCCGTGACT33.614457831325301No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG33.614457831325301Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33.614457831325301Clontech Universal Primer Mix Long (96% over 26bp)
GACCAGTCCATTCTATGCACAGGCGAGTCTGGAGCCGGGAAAACCGAAAA33.614457831325301No Hit
CTCTCAGTCCCACACAAGGCAGCTGTCTCACACTTTACAAGCTGTGAGAG22.4096385542168677No Hit
AGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT22.4096385542168677No Hit
GTACTTTGCCGACGGCCGCTCGCTGCGCTGCAACACCTTCGACTTCTCCA22.4096385542168677No Hit
TTCTTCAACCTGGCCAAAATCGCAGCTTCGGGCCGACTGGGCCAGGAAGA22.4096385542168677No Hit
TCTTTGTATTCACTGACATGGAAAGATTTCCAATATACTTTGAAGTAAAA22.4096385542168677No Hit
CGCTGGTACTGCTTGGCGTGCTGGCTTTGGCGGGTGCGGGCCTGGCGGCC22.4096385542168677No Hit
ATGTTAATGATGTTTTCTGTAACGAACTGTTTCGAAAGCCCCACATTACA22.4096385542168677No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG22.4096385542168677No Hit
ATCGTCGACATGTACAAGGGCAAGAAGAGGCACGAGATGCCGCCTCACAT22.4096385542168677No Hit
GGTGATCCTCGTCACCCAGGATGTCGGACAGGACGGCGAAGTCCTTGCCT22.4096385542168677No Hit
CGTATCCATCGCGCCGATATGACCGTAAAACAGATCATATTTATCAGTAC22.4096385542168677No Hit
CCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGG22.4096385542168677No Hit
CCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAA22.4096385542168677No Hit
CTCCTGTGGCTGGGCCTAGCCCTGTTGGGGGCTCTGCATGCCCAGGCCCA22.4096385542168677No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGCGTTGAAACCACTGA11.2048192771084338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit
CCTAATACGAGAGGCCCATGACGGTACCGTAAGAATAAGCACCGGCTAAC11.2048192771084338No Hit
CGCTGGTCGTCGGCATCCGGATCAATTTCGCTGCGGCGAAGGCCTGGAGC11.2048192771084338No Hit
GCCGGGAGGAGCCTCTGAGTTCCTCTTCAACCAGATCCTGGTGGAGCTGC11.2048192771084338No Hit
CATAATGATGTACACCCCTGCCAAGATGGCCGATGGGTGCCGGTTGATCG11.2048192771084338No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
GGTTTGGACCCTATAAGTTCATCCAGGACTGTCAGGCCCTGGAAAACTGA11.2048192771084338No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT11.2048192771084338No Hit
AACCGCTCCCGCAGCGCGGTCACCAGCCGGGCGGCGGCGGGCGGCCGGAG11.2048192771084338No Hit
GAAGCATACTTGTAGAAGCAAGGCCAGTCCAGCATCAGAAGGCAGAGGCA11.2048192771084338No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC11.2048192771084338No Hit
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA11.2048192771084338Clontech Universal Primer Mix Long (96% over 27bp)
GTACTTTTTTTTTTTTTATGTTGATACCACTGCTTCCCATGTACTCTGCG11.2048192771084338No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTACACTGCGT11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGTACAGCTGCCCACCCCCAGCACTTGTCAAAGAGGCCCTCAGCAATGT11.2048192771084338No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTTCGCGAGCGTAAACATCTGTAATAATTGGCATGTTTGTAATCTCCT11.2048192771084338No Hit
TCTCACAGGCATTGTTTGCTTTGGTAAGTTCACCATGATCCAGGGCATGG11.2048192771084338No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT11.2048192771084338Clontech Universal Primer Mix Long (95% over 21bp)
ATCTTAAAACCAGAGTTCACTGATCTCACATTTTAAAAAGAAAAAAGTTT11.2048192771084338No Hit
CGACGCGATCGTCCTTGGCGCCGGGGCCGCCGGGCTGCTGTGCGCCGCCA11.2048192771084338No Hit
AGTTGGTCACGTGGTCACCCAATTCTTTGATGGCTTTCACCTGCTCATTC11.2048192771084338No Hit
ACCATAAGAACTTCGCCTTCTTTATGACATTGGGAAGGCCGCCATGCCAC11.2048192771084338No Hit
GGCGCCACCCCGCGGTCATCGGTCACCGGTCGCTCTCAGGAACAGCAGCG11.2048192771084338No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
GTGGCAAACTGTACTTCCCAAAAATGGCCTTAAGAAAGTCTCTCATCTCT11.2048192771084338No Hit
GTGCGGCGATTTCGTCGCCGGTCGACTGACCGCTTCAGGCGTCAGGCAAG11.2048192771084338No Hit
CTCCGAGCTGGCAGATTGAGACCAAGTATTCAACGAAAGTGCTCACTGGA11.2048192771084338No Hit
ATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAG11.2048192771084338No Hit
GTCTTATACACATCTCCGAGCCCACGAGACGAGCCAATAAGGATCTCGGA11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338Clontech Universal Primer Mix Long (95% over 21bp)
GAGGAAGGCGGGAACCTAGGAGGCCTGATTAAGATGGTCCATCTACTGGT11.2048192771084338No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC11.2048192771084338No Hit
CCGAGCCCGTCTCGCCGCACACGATGATGACCTGGTGCCGGTCCATGGCC11.2048192771084338No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)