FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44.819277108433735Clontech Universal Primer Mix Long (96% over 26bp)
AACCCGTGACGTCGAAGTTGATGCGGATGAATTTGCCGAATCGTGAGGAG33.614457831325301No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA33.614457831325301Clontech Universal Primer Mix Long (95% over 22bp)
ATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACAT33.614457831325301No Hit
CTCGTAGAGGCCGTGCGCCATGTCGACATGCTGCAGCCCGCCACCGCAGA33.614457831325301No Hit
ACCCTGAGGTGCTGGGCCCTGAGCTTCTACCCTGCGGAGATCACACTGAC22.4096385542168677No Hit
CACCATAGCATGCTGGTTGTAGTTGGTGCTCACCACTCGGACGAGGTAAC22.4096385542168677No Hit
GAACTCGGCTTCGGCATGGGGTTCGTGCTGATCTTCAGCTTCATCAGCCA22.4096385542168677No Hit
ATGAAATAGCCACCGCCATTGCGCTTGGCCTGTTCGGTTCTGTCGACGCC22.4096385542168677No Hit
GCCCAGATGTCGGCGCGCGCGGTCGTGCACTGCACCAGCGCGCGTTCCTG22.4096385542168677No Hit
CAGGCGATCTGCACCACCACCCTTGGCACCAAGGACGCGGAGCAGATGAT22.4096385542168677No Hit
CCTTCTTGGTGTTTTCGGTTTTCCCGGCTCCAGACTCGCCTGTGCATAGA22.4096385542168677No Hit
GCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCC22.4096385542168677No Hit
ATTTTATACACATATACAATTCTATGTCATTTGTTTTTACTTCAAAGTAT22.4096385542168677No Hit
GGGGGCGATTTGCGGGTAGTAACGCTCACGGCAATGGCTGCACAGCATCG22.4096385542168677No Hit
ATACGAATGGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAG22.4096385542168677No Hit
GGCAGTCTGCCCGACGGCGTCAGCGGCGGGCTGGCACCGATTTCGACGCC22.4096385542168677No Hit
GCCACCGCACCTGGCCTTCCCTGCTTCCTCTCTAGAATCCAATTAGGGAT11.2048192771084338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGTAAAAACCAA11.2048192771084338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATA11.2048192771084338No Hit
GGGTGGAGAGGTTAAAGGAGCCACTTATTAGTAATGTTGATAGTAGAATG11.2048192771084338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACATAAAAAAAAAAAAAG11.2048192771084338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAACAACGCAG11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCATGCGAGTCAGCCTTGAGCTGTAAAAACAGCCCAAACGCGGAATATC11.2048192771084338No Hit
CGGTGTGGATGTTGGTGAAGTTGCACCGCCCCCCGCCCGAGATGACGATC11.2048192771084338No Hit
CTCCTTAGTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGA11.2048192771084338No Hit
GAAGGGGAAAGTCAGACTTCTCTGGCAGCCACAGCAACTTCTGTCCATTC11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTATAGCCATTGTACATT11.2048192771084338No Hit
ACCAACTACTCTTGCAACTCGCGAGGTATATCGGAATAACTGGATGGTAG11.2048192771084338No Hit
CGGTGATGGCGCGCGAGCTGATCAGCCAGCGGCGCCCTCCTACCGGCAAG11.2048192771084338No Hit
AAAATACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2048192771084338No Hit
GTACACGTGCTGTGCAACAGCGAAGACATCCGCCTCAGCCCCTTCATGGC11.2048192771084338No Hit
AGCCTCAACCTCCTGGGTTCAGGTGATCCTCCCACCTCAGCCTCCTGAGT11.2048192771084338No Hit
CCGCTGGCCTGACTTGGCGAAGGCCCTGGCATGCGGGGGTTTTCATGCGG11.2048192771084338No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
CGGTAGAGGGGGATGGAATTCCGCGTGTAGCAGTGAAATGCGTAGATATG11.2048192771084338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCAAAAAAACCCCCC11.2048192771084338No Hit
CGGTGATGGCGCGCGAGCTGATCCGCCCGCGGCGCCCTCCTACCGGCAAG11.2048192771084338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGAAAACCAA11.2048192771084338No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA11.2048192771084338No Hit
AAGCTGGGCCGCACGATCGGCTATCCCACCGCCAACATCGACATGGCGAA11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACAGCATCCC11.2048192771084338No Hit
GCCTAACACTACGCCCTACCTCACATTCCTATTGACATCTGCTGCAGATC11.2048192771084338No Hit
CCTCTGAGGTGCCTGCTGGCATCCTCTCCCCCATGCTTACTAATACATTC11.2048192771084338No Hit
CCTCTACACCCTGGACTACTACCTGCGGCTGGCCGAGGAGATGGTCGGCG11.2048192771084338No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT11.2048192771084338No Hit
GACTGATGACACGCATAATCAAATTGAAGTAATTAAATTATTTGTATTTA11.2048192771084338No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTACCCATGTACCTGTCTCTTA11.2048192771084338Clontech Universal Primer Mix Long (96% over 27bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGTAAAAACCAAC11.2048192771084338No Hit
GTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTC11.2048192771084338No Hit
TTATGGGAGACCAACCTTGTGCCTCCGGGAGATCCACTCTCCCACCTGGA11.2048192771084338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAA11.2048192771084338No Hit
GCAGTGGTATCAACGCAGAGTACGCTTCCCATGTACTCTGCGTTGATACC11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCCCATGGAGATGTGGAAGTAGAAGGGGAAGAGTTTGCTCTGCACTAGTC11.2048192771084338No Hit
GGTACAGGGCGCTGTGGGCAGGACCCTTTGGACTCCTCAAGTCTCCGACT11.2048192771084338No Hit
GCGCCTTCCACGACGCTGCGGTATTGCTTCTGCAATTCGTCTTCGCCGGC11.2048192771084338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTAAAAACCGCCCC11.2048192771084338No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)