FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGGAAGCGCCCGGCGCGTAAGGGATCGCAGACGCCGTTCGTGAAGAA36.666666666666667No Hit
TCGTGGTAGCGCGCTTCGTCGCGCGAGGAGAATTGCAGGTTGCCGTTTAA36.666666666666667No Hit
CAACAGCGGCCTCCGCCCGTCCGCCGGCCAGCGCCTGCTGTTGACGCAGG24.444444444444445No Hit
GGGGCGCCCCCGAGACCTGGCTGTGGGGCGCCGGCGTGCTCATCGCGGTC24.444444444444445No Hit
GATTATTGCAGCAGCTTCCTAACTGGTCTCCCAGCTTTCACATTGCTCCC24.444444444444445No Hit
GTATGAAAACATGAGTGGGGGGTTGGCCTAGATGGTAAACGAGGAGAGGA24.444444444444445No Hit
GAATTACTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGTGCC12.2222222222222223No Hit
GTACTGGCTGTCCGTCTGCGCGCAGCCGACCGAGCAGGTCGCGGCGCTGC12.2222222222222223No Hit
CTCCAAAAGTGCTGGGATTACAGGCGTGAGCCACCACGCCTGGCCATGAC12.2222222222222223No Hit
GAGGATAATAGTACCTACATCAAAGAGTTCCATGAAGCAATGCATGGGAA12.2222222222222223No Hit
GCATCCCAGGGATGAAGCCCACTTGATCATGGTGGATAAGATTTTTGATG12.2222222222222223No Hit
GCCCTATCGCTGTCATGACTGCTGCAACAGTGGGCTGTAAAACCATGCAG12.2222222222222223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGT12.2222222222222223No Hit
GATCAAGCCACTGCATTATAGCCTAGGCAACAGAGCCAGACCCTTTAACA12.2222222222222223No Hit
GGAATGGAATGGAATGGAATCAACCTGAGCGGAAAGGAATGGAATGGAAT12.2222222222222223No Hit
ATTTTGCCGAGGCTAGTCTCGAACTCCTGGCCTCAAATGATCAGCTTGCC12.2222222222222223No Hit
CCTACGGGACGTTCGTGGCGGGGGTCACGCGGGAGACCATCTCCGACGTG12.2222222222222223No Hit
ACTCCACCCTGAGTGACAGATTGAGACGCTGAGAAAAAAAGGTACAATCT12.2222222222222223No Hit
CCCCTATCGCTGTCATGACTGCTGCAACAGTGGGCTGTAAAACCATGCAG12.2222222222222223No Hit
GCTTCTGGCGGTCCGGGGATGGACCCTGTTGGAAGGACAACTTCGAGGAA12.2222222222222223No Hit
ATATTCGCCGCCTTTCACAATGTTGTAGATCTGCAAACCACGGTATCTTT12.2222222222222223No Hit
ATTACCTGGAGCAGCTCCGCTCAGGGCAGGCAGCAAGTTAACAGCGCCTG12.2222222222222223No Hit
GTTTTTTTGTTTGTTTGTTTTGAGACAGAGTCTCGCTCTGTTACCCAGGC12.2222222222222223No Hit
CGTTCGCGCGAGGACGGGCGCGACCAGTCCGATGAACCCGATCGCGCCGC12.2222222222222223No Hit
ATGCTGATTCGCTGTCAAGGGCTAGATGTCTATGATCATTAAGCTATTAG12.2222222222222223No Hit
ACTATTTTCAGTCTATCTCAAACTCTCTCAAAACTGAAGCAGGTGCTAAC12.2222222222222223No Hit
CGTATACAGTGTGCTCACTAGCAATGTCTTTTTTTTTTTATTATACTTTA12.2222222222222223No Hit
GATTTGAAACACTCTATTTGTGCAATTTGCAAGTGTAGATTTCAAGCGCT12.2222222222222223No Hit
GACTTACTTGAATATGTGCATTCAGGATTTATTTTCCAAGTTATTGTTTT12.2222222222222223No Hit
AGTCTTAGCGGCTTTTGCTAATTAATTTGGGCAGGTCGAAATCTAGTATT12.2222222222222223No Hit
CTGCCCTGCTCGGCTACGTCCCGGCGTCGCGCGAGCTCGCCTGGCCGCTG12.2222222222222223No Hit
AGGCGGTGGCATCGTCAACAATTGCCACGGTCTTGGCGCCCAGGGTCTTG12.2222222222222223No Hit
GCATTTCTTTGCTTCTCATTTCTTCATGTGTTTGACATTTCTCCTAGCTC12.2222222222222223No Hit
GGAGACTTAGTGCCCAGCTCGGGCCTGCCAGGCTCCAGAGCTCAGGGTTT12.2222222222222223No Hit
CCTCAGCCTCCCAAAGTGCTGGGATTACAGACACGAGCCACTGCACAGAA12.2222222222222223No Hit
GAATAATCTCTCTAGCATCACATAAAAAGATTGCTAATGATGGAATTTTG12.2222222222222223No Hit
ACGGCACGTAGTGCGGAAACGTCGGGGCCGGGATTCGAACCCAGGTTCTG12.2222222222222223No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)