FastQCFastQC Report
Tue 10 May 2022
EGAF00003625855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117873
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.2816590737488653No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGCCAGACATATCTCGTATG3120.2646916596676084No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3050.2587530647391684No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGCCAGACATATCGCGTATG2200.18664155489382642No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2110.1790062185572608No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2080.17646110644507224No Hit
ATACACATCTCCGAGCCCACGAGACGTTGCCAGACATATCGCGTATGCCG1990.1688257701085066No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1980.16797739940444378No Hit
ATACACATCTCCGAGCCCACGAGACGTTGCCAGACATATCTCGTATGCCG1970.1671290287003809No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1930.16373554588412953No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1800.15270672673131253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.14931324391506112No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1650.13998116617036982Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.13828442476224412No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1540.1306490884256785No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1460.12386212279317571No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1460.12386212279317571No Hit
ACACATCTCCGAGCCCACGAGACGTTGCCAGACATATCGCGTATGCCGTC1430.12131701068098717No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1420.12046863997692432No Hit
ACACATCTCCGAGCCCACGAGACGTTGCCAGACATATCTCGTATGCCGTC1410.11962026927286147No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1410.11962026927286147Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1400.11877189856879862No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1400.11877189856879862No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1300.11028819152817015No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1190.10095611378347882Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)