FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003625908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2810.566037735849058Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT166.037735849056604Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT114.150943396226415Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG103.7735849056603774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG103.7735849056603774Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC83.018867924528302Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC51.8867924528301887Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT51.8867924528301887Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG41.509433962264151Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG41.509433962264151Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTCGATGGCGTTACTCATCGGCGGCGTGTCGATGGGCGATCAGGTCA41.509433962264151No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT41.509433962264151Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC41.509433962264151Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA41.509433962264151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG31.1320754716981132Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31.1320754716981132Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG31.1320754716981132Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG31.1320754716981132Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31.1320754716981132Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGCATGTGTCTTTATAGCAGCATGATTTATAATCCTTTGGGTATATA31.1320754716981132No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC31.1320754716981132Clontech Universal Primer Mix Long (96% over 25bp)
ATCGTGTGCGGTTTCGCCCGCATCGACGGCCGCTCGATCGGCGTGGTCGG20.7547169811320755No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.7547169811320755Clontech Universal Primer Mix Long (95% over 22bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.7547169811320755Clontech SMART CDS Primer II A (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
GATCGGCTTATGACGTATGTGACGCAGCTCTTAGCGCAATGTATGTGCTG20.7547169811320755No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC20.7547169811320755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.7547169811320755Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGCAGAGTACATGG20.7547169811320755Clontech SMART CDS Primer II A (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.7547169811320755Clontech Universal Primer Mix Long (100% over 23bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.7547169811320755Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.7547169811320755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAC20.7547169811320755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGAAAGCAGTGGTATCAACGCAGCAGTGGTATCAACGCAGAGTAC20.7547169811320755Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.7547169811320755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACACATG10.37735849056603776No Hit
CTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTAC10.37735849056603776Clontech Universal Primer Mix Long (96% over 28bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.37735849056603776No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTAACCATGTACTCTGCGTTGATACCACTGCTTAACAATTAAAAATCC10.37735849056603776Clontech SMART CDS Primer II A (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA10.37735849056603776No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATTACTATGCGTTGATACC10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACCTGCTTCCC10.37735849056603776Clontech SMART CDS Primer II A (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCCTGTCTCT10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACA10.37735849056603776Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTGTCTC10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCCCCAAGTACTCTGCGTTGATACCACTGCT10.37735849056603776Clontech SMART CDS Primer II A (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATCTGTCTCTTATAC10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
CCCTGAAGCTTCACCGGCGCAGTCATTCTCATAATCGCCCACGGACTTAC10.37735849056603776No Hit
GTTGATACCACTGCTTCCCTTGTACTCTGCGTTGATACCACTGCTTCCCA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGAAACACTGGTAT10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCC10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACGGCCCCACCCAGCACTGCGTCGAAGTTGCCGCGGAAGTCGCGAGAC10.37735849056603776No Hit
CGGCAGTCTTGCTGCGGGTTCGAATGTCATGGGAGTGCGCGATGTGGATT10.37735849056603776No Hit
TCTCCGTCCAGCAGCCGGCACAGGTCGAGCCGGCGGTCGCGGCGGTGGCA10.37735849056603776No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTATACCACG10.37735849056603776No Hit
GTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT10.37735849056603776No Hit
GTACATGGGTAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.37735849056603776Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTACACTATAGTCATCGTCCATAACACGATCTAGTGAAACCTTCAAGCA10.37735849056603776No Hit
GCAGACTACATGGGAAGCAGTGGTATCAACGCAGAGTACACTGTCTCTTA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GCTTAAAACTGTCCTTTAATGAAAAGAAAGCCAGTTATGAAAATCTGCAT10.37735849056603776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTCATGGGAAGCAGTGGTAT10.37735849056603776Clontech Universal Primer Mix Long (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTTGATA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAA10.37735849056603776No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCTTAACCATGTACTCTGCGTTGATACCACTGCTTAACCATGTAATCTGC10.37735849056603776Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTTTGAT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
TGTGGAACATCCCCCTGCGCAAGAGCTTGAACAGAATACGAAAGACTTCG10.37735849056603776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAGTGTTA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCAATGTACTCTGCGT10.37735849056603776Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGGGGGGCAAA10.37735849056603776No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGGAAGC10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAAAAAAT10.37735849056603776No Hit
GCCCACGGGCACGTTCGTGGCGGTGACGCGAAGGCGCGCGCCGCACGAGT10.37735849056603776No Hit
CCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA10.37735849056603776No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGAT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCCATGGAAAGCAGTGGTAT10.37735849056603776Clontech SMART CDS Primer II A (95% over 24bp)
GCTTCTCATGTACTCTGCGTTGATACCACTGCTTCTCATGTACTCTGCGT10.37735849056603776Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGAAGTGGAA10.37735849056603776Clontech SMART CDS Primer II A (100% over 26bp)
TGGGAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.37735849056603776Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTAC10.37735849056603776Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGT10.37735849056603776Clontech SMART CDS Primer II A (96% over 25bp)
CATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGTACT10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.37735849056603776No Hit
CGGCTCGTCGTCGCGCCACCGATCAGCAGCGGGATTTTCTTCACGCGGAA10.37735849056603776No Hit
GGCTTTAATCAACCCTATTGTGCCAATTGAGATTAAATCTTCTGTGTCTT10.37735849056603776No Hit
GGAATAAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAA10.37735849056603776No Hit
CGCAGAGTACTTTTTTTTTTTTTTTGCGTTTATACCACTGCTTCCCATGT10.37735849056603776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTAATGGGAAGCAGTGGTAT10.37735849056603776Clontech SMART CDS Primer II A (96% over 26bp)
TAACCGAACAACCCGACGGGTAATCTGTTCGACGCGGCGGACATGGACGC10.37735849056603776No Hit
CGACACGCACCGCCGCATCGGCGCCACTCGCCTGAACGGGATCGACGCCG10.37735849056603776No Hit
GCTCGCTGCCGCTGGCAGACGAGCGCCGCATGCCTGTGAGCGAAGACGGT10.37735849056603776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATAACCCA10.37735849056603776No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTAAAAAAA10.37735849056603776No Hit
GCTTCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGGGAAGCAGTGGTATCAACGCAGAGTACAGGGAAGCAGTGGTATC10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGAAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.37735849056603776Clontech SMARTer II A Oligonucleotide (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)