FastQCFastQC Report
Tue 10 May 2022
EGAF00003625911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27571
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2500.9067498458525262No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1440.5222879112110551No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1040.3772079358746509No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1020.3699539371078307No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT900.32642994450690943No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT840.3046679482064488No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA760.27565195313916796No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA750.27202495375575786No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA740.26839795437234776No Hit
ATACACATCTCCGAGCCCACGAGACCGCACTCCATCAATCTCGTATGCCG710.25751695622211745No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA690.2502629574552972Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG640.23212796053824672No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA630.2285009611548366No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG630.2285009611548366No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT600.2176199630046063No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA550.19948496608755578No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC530.19223096732073555No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG480.17409597040368505No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG470.17046897102027492Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACCGCACTCCATCAATCGCGTATGCCG460.16684197163686482No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC460.16684197163686482No Hit
ACACATCTCCGAGCCCACGAGACCGCACTCCATCAATCTCGTATGCCGTC460.16684197163686482RNA PCR Primer, Index 36 (95% over 21bp)
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC460.16684197163686482No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG450.16321497225345472No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA450.16321497225345472No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC450.16321497225345472No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT430.1559609734866345No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG430.1559609734866345No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC430.1559609734866345No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC410.14870697471981428No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC410.14870697471981428No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG410.14870697471981428No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC390.1414529759529941No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT390.1414529759529941No Hit
ATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCC390.1414529759529941No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT380.13782597656958398No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT380.13782597656958398No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA380.13782597656958398No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA370.13419897718617388No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC360.13057197780276378No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG350.12694497841935368No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT350.12694497841935368No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC350.12694497841935368No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT350.12694497841935368No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA330.11969097965253345No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC320.11606398026912336No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG320.11606398026912336No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA310.11243698088571326No Hit
GTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACA310.11243698088571326No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG310.11243698088571326No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT300.10880998150230314No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG300.10880998150230314No Hit
GCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGA300.10880998150230314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.10518298211889304No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG280.10155598273548293No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG280.10155598273548293No Hit
ACACATCTCCGAGCCCACGAGACCGCACTCCATCAATCGCGTATGCCGTC280.10155598273548293No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC280.10155598273548293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)