FastQCFastQC Report
Tue 10 May 2022
EGAF00003625991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003625991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52028
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.44014761282386405No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1810.34788959790881835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.3248250941800569No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1330.2556315829937726No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCGATGGCAGCATCGCGTATG1110.21334665949104326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.19220419773967864No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCGATGGCAGCATCTCGTATG950.1825939878526947No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA830.15952948412393328No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA760.14607519028215576No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG740.1422311063273622No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA690.13262089644037825No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT670.1287768124855847Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA670.1287768124855847Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA640.12301068655339432No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA610.11724456062120396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG590.1134004766664104No Hit
ATACACATCTCCGAGCCCACGAGACTCCGATGGCAGCATCGCGTATGCCG590.1134004766664104No Hit
ATACACATCTCCGAGCCCACGAGACTCCGATGGCAGCATCTCGTATGCCG560.10763435073422004No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.10379026677942646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)