FastQCFastQC Report
Tue 10 May 2022
EGAF00003626085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838774
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT18270.21781791042640805No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT17520.20887628848772136No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA17470.2082801803584756No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC14890.17752100088939332No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT14370.1713214763452372No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC14030.16726794106636592No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG13870.1653603950527794No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC13790.16440662204598616No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC13450.16035308676711485No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT12710.1515306864542773No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC12380.14759637280125515No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC12320.14688104304616023No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC12230.14580804841351783No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG11740.1399661887469092No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA11550.1377009778557752No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC11440.1363895399714345No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA11270.13436277233199886No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA11210.13364744257690392No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT11110.13245522631841233No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG10980.13090534518237332No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA10870.1295939072980326No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT10810.12887857754293766No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC10770.12840169103954102No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG10680.12732869640689864No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT10640.126851809903502No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT10530.1255403720191613No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG10360.12351360437972565No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA10250.12220216649538494No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC10200.12160605836613915No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC10200.12160605836613915No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC10190.12148683674029No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT10160.12112917186274254No Hit
GTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGA10110.12053306373349675No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10060.11993695560425097Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT9900.11802940959066448No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT9840.11731407983556955No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT9790.11671797170632375No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT9690.1155257554478322No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA9650.11504886894443556No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC9610.11457198244103894No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC9570.11409509593764233No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA9320.11111455529141342No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG9250.11028000391046934No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG9150.10908778765197777No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT9070.1081340146451845No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCCGTAAGTAGGAGAGTGATAT8990.10718024163839128No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC8970.10694179838669296No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC8820.10515347399895562No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT8820.10515347399895562No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC8730.10408047936631322No Hit
GTACATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAA8720.10396125774046407No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG8700.10372281448876575No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA8680.10348437123706743No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA8630.10288826310782165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)