FastQCFastQC Report
Tue 10 May 2022
EGAF00003626104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24557
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA2430.9895345522661563No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1740.7085556053263835No Hit
GTAGTAGACAGCCTTGCTCGTGGTTGTTGCGGACGTTGCGCTTCCGGCCG680.2769067882884717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.2606181536832675No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA540.21989656717025696Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGG460.1873192979598485No Hit
GTCTACTACAACGCCCTGTTCGACGTGCCCAACGACCACGGAATCCTGCG370.15066987009813904No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT370.15066987009813904Clontech Universal Primer Mix Long (96% over 26bp)
ATTATAAATAGTGCTGCTTTGCTTGGTGCGGTTGCTCACACCTGTAATCC360.14659771144683797No Hit
GTCCATAACTCTCTCTTAGAACCCTCAGTCTCCATAGAAGAAGCCCAGTT340.13845339414423585No Hit
GTCTTGTTCTGTCCCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCAA330.13438123549293482No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT330.13438123549293482No Hit
GTACATGGGTGAGCCAAGACCCCACCTTTGCACTCCAGCCTGGGCAACAA320.13030907684163376No Hit
GTATGTATACATGTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGC290.11809260088773059No Hit
GTGTTGGAAGTGGGGGCTAGTGGGAGGTGTTTGGGTCATGGGGACAGATC280.11402044223642954No Hit
CTCTATTGCCATGTGACATGCCCGCTCCCATTCGCCTTCTGCCATGATTG270.10994828358512848No Hit
ATGTATACATGTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGCAC270.10994828358512848No Hit
CTCCCAGACATTGCAGATGCTGTCATACCTTTCCATGGAGCCCATGAGCT260.10587612493382743No Hit
CCATAGCGCCGGACCCTGCGGCACGGCGGCATCGGCCCCGACCGGTTCAC250.10180396628252637No Hit
GTCTACATCTTCTCCCTGAAACTCAATCAGTGGAGGCTGGGGGAGCCCAT250.10180396628252637No Hit
GTAGCAGAGTGGTCCCCAAGAGATAAAAAGTCTTTGAACAGAATCCAGAA250.10180396628252637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.10180396628252637No Hit
GCCCATAAGAACTTCATCTTCTCCATCGGGGGGATTGGAGAAGGGCAGGA250.10180396628252637No Hit
GTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGCACACATACTCAT250.10180396628252637No Hit
CCATAGAAGAAGCCCAGTTTCCCTGAGGCTATCATGCTGGAGAGATCACA250.10180396628252637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)