FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCCTGCAGAAGACAGGTCCTGCCAGCAACGGGAACCAGAATTTGGG35.88235294117647No Hit
GGGTGTCTGTCCAGTTTTGCACAGGCTCCTCTAGCTCCTGCACCATTTCC23.9215686274509802No Hit
ACTGCATACTTGGGAGAGGGGACATCAGGGAAAATGGGGACTCTGCACTC23.9215686274509802No Hit
TCCATTGACACCCAGTGCGGATGGTCGATCCAGATCTCCCGGGAGTCCTC23.9215686274509802No Hit
GGCTACTCTCAAACTCCTGGGCTCAAGCGACCCTCTCACCTAGGCCTCCC23.9215686274509802No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA23.9215686274509802No Hit
GGCCACAGTGAGCAGTGCCCTTGGCTGTATTCCCCAGGGTTCTGTATGTT23.9215686274509802No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTACAC11.9607843137254901Clontech SMARTer II A Oligonucleotide (95% over 21bp)
ATGCATTCTATGCCCTTTGATTTAGTCTGGGGAAAGAATAAACATTGCCA11.9607843137254901No Hit
CCAATATGAAACTACTTTTTAGAGAGAAAATGTGCTGTTCAGTAACTGTC11.9607843137254901No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG11.9607843137254901No Hit
TCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGAT11.9607843137254901No Hit
CATACTAAATGGGCAAAAGCTGGAAGCATTCCCTTTGAAAACAGGCACAA11.9607843137254901No Hit
GTTTCAAATTTCAGGTTACTGCTGCTTTCACTGTGATTGTTTTTGTACAG11.9607843137254901No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGC11.9607843137254901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTACT11.9607843137254901No Hit
GAACCGGAGAATGGAAATCCGTAGGTTTTGAAAAACAGATAAGCGGAGGT11.9607843137254901No Hit
GTCTGCATCTCACTGTATTCCCCGCAGTAGTTTATGCGTTCATTGTATAC11.9607843137254901No Hit
GTTGATGTTGGATAAGAGAATTCTCTGCTCCCCACCTCTAAGTTGCCAGC11.9607843137254901No Hit
ACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTTATA11.9607843137254901No Hit
GACTAGGAGTGCTACTTTGTTAAACTTCCTGTTTAACAAAGGTCAGGATC11.9607843137254901No Hit
ATCCGCTGACCAACGGCGGCGAGCTGGCGGCGCTGTTCTCGTTCGTGTTC11.9607843137254901No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCGAGTACATGGGAAGCA11.9607843137254901Clontech Universal Primer Mix Long (95% over 21bp)
CCCACAGAAAGTCGGGGATGTCTGTCATTCCTCTCGTGACCAGGCTGAAG11.9607843137254901No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGG11.9607843137254901No Hit
TGCGCGAGATCCTCTTCAATTGCTCGGTGGTGTCCACCTCCTGCACGTGT11.9607843137254901No Hit
ATGATTGGGTCCCCAAGGCACGAACCATTTTGTCTTGTGATGGTTCTTAC11.9607843137254901No Hit
ATCCAAAATATACATAGAGCTCTTAAATTCAGCAATAAGGGCTATCTAGG11.9607843137254901No Hit
GCCTTCTCGACCGCCTGGTGCGCCGCTACGCGGAGACCAAGGACTCGGGC11.9607843137254901No Hit
ATACAGGACAAGGCGTTGATCAACTCGCTAACATTATTCACCAAATCCGT11.9607843137254901No Hit
TGGCGCTTTAAAGGGATCGCAGCACATGCCGCGAATTCCCCTCATTTGGG11.9607843137254901No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT11.9607843137254901No Hit
GGGAAAAGTATCTCAGGAAATGATTCCAAAACATTTTCAAAAGACATCGT11.9607843137254901No Hit
CATCTTATGTGCAAAGAAATATGGGTTTTATTCCAAAACTGAAAGCCTCT11.9607843137254901No Hit
GTATGGGCCGTGGCTGGGGTTCAGGCCATTTGGAGCTGGCAGCCTCACAG11.9607843137254901No Hit
AGTGTCCACTGAGCTTGGAGGGGGTGGAGCCGGGACCAGCCCCCTAGGAA11.9607843137254901No Hit
GGGCATGGAAGACTGCGGAGAGGAACACGGGAAGGTGGAGTAGAGAACAG11.9607843137254901No Hit
TAACTTAACTTTAATCTTTTTCAAAATGTTGAATGTTTCCCAGCTACTGC11.9607843137254901No Hit
AACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATAT11.9607843137254901No Hit
ACCAGCCCTACGACGCCCACCGGAATCGAGCCCAGGATGATTCCCCAGCC11.9607843137254901No Hit
GGAAGGGGGTCCCGCCCCGTCACGCGGCTGTCTCTTATACACATCTCCGA11.9607843137254901No Hit
AGATATGACAGGTGTACTGGTACTCCATGACCATGACAGAGCCCTATTAA11.9607843137254901No Hit
GGGTTTCTCCATGCTGGTCAGGTTGGTCTCGAACTCCTGACCTCAGATGA11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)