FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCTAACCTGATGCAGGATGCAGCTTCCTGATGCAGAACCACAACACG35.88235294117647No Hit
ATTTTAACCAGATTTTCTCAGGCCTTTTTTGGATACCTTTAGTAGTTAAC23.9215686274509802No Hit
AGTGCAGAGTCCCCATTTTCCCTGATGTCCCCTCTCCCAAGTATGCAGTC23.9215686274509802No Hit
ATTCAATATGACTAATGTCCTTATAAGAGGTGCAAAGTATTGTGTCAAAT23.9215686274509802No Hit
TCTTGTCTGATCAAAGCAGTAAGTATGAAATACAGAGAGGACACTGAAAG23.9215686274509802No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT23.9215686274509802No Hit
GTGCCGTCCCGACGCTGCCTCTCCCTCGACGATTGGGTAACCGGAGGATC23.9215686274509802No Hit
GGATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACA11.9607843137254901No Hit
CTGGAAATCAGGGAAACTTCTTTTCAGATGTTACCAATAAGCAATGAACC11.9607843137254901No Hit
GTGTGTCTGTGTCTTTGACTGATGGACACCCAGAAGCCAAACTTTTTCTT11.9607843137254901No Hit
CTTCATAGAAACCAAACACTTTCTGAAATTCCCTTTTTTTCCCAGCTTCT11.9607843137254901No Hit
CCTTAGTTCGCTCACCCCTGCTTTTAGTTTTTCCTCAGTGGAGAATGAAA11.9607843137254901No Hit
GCTCTGCTCTTTTCACTTTCACACCTCTTTGTCTCTCTCCTTCTCAAAGG11.9607843137254901No Hit
CTACACGCTGGCCGCCCAGATCTCAGAGCGCATCATTGTGCGGGCCTCCA11.9607843137254901No Hit
GTCTCAGACAGCCTGGCAACAGCACTTCTGCAGAAACTGTAGGGCTAATT11.9607843137254901No Hit
TCTGTGGGCAATGACACAAAGAATTGACACAGGATATGGCACGGGGGGAG11.9607843137254901No Hit
TCTGAGCAGGTTGCTCCACAGGTAGCTCTAGGAGGGCTGGCAACTTAGAG11.9607843137254901No Hit
GCATTGGGCACCCAAAGTGCTGGGATTACAGGTTTGAGCCATTGCGCCTG11.9607843137254901No Hit
GTACTGTGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGT11.9607843137254901No Hit
TTACTGAACAGCACATTTTCTCTCTAAAAAGTAGTTTCATATGGGCTGTC11.9607843137254901No Hit
CCGCGTGACGGGGCGGGACCCCCTTCCCTGTCTCTTATACACATCTGACG11.9607843137254901No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC11.9607843137254901No Hit
GTTTGTAAGCTGTTCTGCTATTTCCATTAATTGCCAAGCGAGGTCAACTC11.9607843137254901No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGAGTTGATAACAAA11.9607843137254901No Hit
GATTTTGTATCCTGAGACTTTGCTGAAGTTGCCTATCAGCTTAAGGAGAT11.9607843137254901No Hit
GGACTATAGCGCCAATGCCATTGCCTTCTATTCCTACACCTTTTCCTAGG11.9607843137254901No Hit
GTTGGGCTCGATACGGAAGACGAGCCGGGCGCGACGCGCCCGGGCCGATA11.9607843137254901No Hit
TTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAA11.9607843137254901No Hit
CTCCATAACCATAAGAAATAGATTTCTATATAAATTGCCTAGTCTCGGGC11.9607843137254901No Hit
CATTTCGGGGGCGCGGATAAGATAAAGCACTTCTGCCTGCGCCTGGACCA11.9607843137254901No Hit
AGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAACA11.9607843137254901No Hit
ATGTTTTTCAGGATTGAAGAAAGGTATGAGTTCTTAGCATGATAAAAAAG11.9607843137254901No Hit
CCCTAAAACATTTATTTCAAGGAGAAAAGAAAAAGGGGGGGCGCAAAAAT11.9607843137254901No Hit
GTGCAAGGGTCTGGCCCACAAAGAAGACCGTGACGACCCGGACTACACCC11.9607843137254901No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11.9607843137254901Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11.9607843137254901Clontech Universal Primer Mix Long (95% over 22bp)
CTCCCACCTCAGCCTCCCAAGTAGCTATGGCTACATGTACGTACATCTCC11.9607843137254901No Hit
GCCAATAAAGAAATACATTAGTTTACACCATTTCCCCATATCATGGCCCC11.9607843137254901No Hit
TCACAATAGATACAAATTGTTTTTCTATTCTTCACCTCCGCTTATCTGTT11.9607843137254901No Hit
AATTAGGGCGTGGTGGTGCGTGCCAGTAATGCAGCTACTCGGGAGGCTGA11.9607843137254901No Hit
CTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTA11.9607843137254901No Hit
CAGCCTGGAAGATAGATGTTAAAAGACAGCTGTGATTAGGACAAAGCACG11.9607843137254901No Hit
GTCCATGGGATTCCAGGCGATCCTCACCAGTCTACACAGCAGGTGGAGTT11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)