FastQCFastQC Report
Tue 10 May 2022
EGAF00003626121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374009
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6700.17914007416933816Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5640.1507985101962787Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.14250993960038394No Hit
ACTCTAGACTGATACCTCCTCATGAAATTTCTGCAGACGTCGCGCCATCG3810.10186920635599679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)