FastQCFastQC Report
Wed 11 May 2022
EGAF00003626138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658451
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG310080.8475718275302854No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG262350.7171067755178353No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG260360.7116673149373874No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC250880.6857547087551535No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC206940.5656492324210438No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA180390.49307753472712906No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT149070.40746753202379915No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT146430.4002513632135568No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA139430.3811175822773081No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC129840.3549043023946474No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC117730.32180286137493713No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG116110.3173747577868338No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC112970.30879189033828797No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG100880.2757451172641099No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT100860.2756904493185777No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA96080.2626248103363965No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG95840.2619687949900108No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT92640.25322192370486857No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA90230.24663443626824574No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT89520.2446937242018548No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA82730.2261339566936936No Hit
CATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAG81190.22192452488771888No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC80990.22137784543239747No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT80300.2194918013115387No Hit
GTACATGGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGG80160.2191091256928137No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG79630.21766042513621203No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG77240.2111276056451214No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT75910.20749218726723415No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG73970.20218939655061663No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC72620.19849931022719725No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA71460.19532856938633317No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA71430.19524656746803495No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC71130.19442654828505287No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT70790.1934971932110065No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT69610.19027178442461032No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC69500.18997111072418355No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT67290.18393030274288216No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC66090.18065022601095382No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG65070.17786216078881473No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT64930.17747948517008974No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT64670.17676880187817193No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC64120.17526543337603812No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG63140.1725867040449633No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT62710.1714113432160223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62320.17034531827814559No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA61700.16865061196664927No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGACACAC60050.1641405064602478No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC58760.16061442397342482No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG57320.1566783318951108No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA55350.1512935392601951No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC55320.15121153734189688No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC53380.1459087466252794No Hit
ATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC52230.1427653397571814No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC52170.14260133592058497No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC52120.14246466605675462No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC51050.13953993097078515No Hit
GCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTT51020.13945792905248697No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC49710.13587717862013185No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC49660.1357405087563015No Hit
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG49150.13434647614523196No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA48600.13284310764309812No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC48340.13213242435118033No Hit
TATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAA46990.12844233802776092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46230.12636495609753964No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA46180.1262282862337093No Hit
GTACATGGGAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG45600.12464291581327726No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC42570.11636072206515817No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT41950.11466601575366187No Hit
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA41350.11302597738769768No Hit
CTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGT41110.11236996204131201No Hit
CAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGG40710.11127660313066923No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTC40510.11072992367534785No Hit
GAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGG39850.10892588147278724No Hit
CACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCT38770.10597381241405175No Hit
GCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCAC38410.10498978939447325No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC38340.10479845158511075No Hit
GTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGC38050.10400576637489474No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC36770.10050701786083781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)