FastQCFastQC Report
Tue 10 May 2022
EGAF00003626153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746158
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.21845239212070366No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG15620.2093390407929688No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG14580.19540097405643309No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA13850.18561752336636478No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG13560.18173094706483076No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA12450.16685474122102825No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC12290.1647104232615612No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT11590.15532903218889296No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG10620.1423291045596241No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG10490.14058684621755715No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT10430.13978272698275698No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT10320.13830850838562342No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC10290.13790644876822336No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT10090.13522605131888957No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG9870.1322776141246224No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA9790.1312054551448889No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA9750.13066937565502212No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT9560.12812299807815503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.12772093846075497No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA9350.12530858075635454No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9230.12370034228675428No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC9150.12262818330702077No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT9120.1222261236896207No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA8590.11512307044888616No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT8570.11485503070395278No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA8440.11311277236188581No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT8390.11244267299955236No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC8060.10802001720815163No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG8010.10734991784581817No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG8000.10721589797335149No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT7810.1046695203964844No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA7760.10399942103415094No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT7710.10332932167181749No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC7630.10225716269208399No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT7570.10145304345728384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)