FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGTGCACTGGGCCTCTCTGAGGTCAGCATATTTGTACTCTTGGAATAT37.5No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT25.0Clontech Universal Primer Mix Long (96% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG25.0Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACC25.0Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTACACCGAGTTCAAGGAGTTCTCCAGGAAGCAGATCAAGGACATGG25.0No Hit
TGTACGGGCCGGGCGGGGCGCGCGGCTCACGGAAGGTGGCTGACGGCCTG25.0No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25.0No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC25.0No Hit
TTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAG12.5No Hit
GGGACTGTTTGTTTACAATGAAGGTAAAAACAGAAGAGAAGTTGCAAATA12.5No Hit
GTAAATGAACTCGAATGGAATCATCATTGAATGGAATCGAATGGAATCAT12.5No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG12.5Clontech SMART CDS Primer II A (100% over 26bp)
AATCATCATCGAATGGAATCGAACGGGATCATCGAAAGGACTCGAATCAA12.5No Hit
GCCCTGGACGGCGCCAAGCGCGACGGCACCCTGGCCCTGGCGGCCGGCGC12.5No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC12.5No Hit
GAATCGGACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGC12.5No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG12.5Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA12.5No Hit
AACGTGTTCATAACAGATTCAGAGAGGAAAACACGTCGAAATCTCCAGAT12.5No Hit
GTTCTAGATATTAGCCCTTTGTCAGATGGATAGATTGCAAAAATTTTCTC12.5No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATGCGTGTAG12.5No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTA12.5No Hit
GAGAAAGGCCAACTTCAAAGACCAGTGAGAACCAGCTGCCCCTGCTTCAC12.5No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12.5Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12.5Clontech Universal Primer Mix Long (96% over 26bp)
TCCACGCTGATGCCCGGCGGCAGGCTGCCGCTGGGCTGCTGGGCCGGAAC12.5No Hit
CATCTTTAATGATGAACTGTCGCCAAGTCAGGTGTAGTCATTGGCAACAG12.5No Hit
TTTTTTTTTTTTGCGTTGATACCACTGCCCCATGTACCTGTCTCTTATAC12.5No Hit
CAAAAATACTTAATCCTGCAATAAAATGTAAAAAGTCACAGATGTTACAG12.5No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)