FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGTGTGTCTGTCATAAAACAAAATGACACCAGTAATTACTTGGTAAT37.5No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25.0Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC25.0Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG25.0Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGACACCCTCACTGGAGACGTCGATCTCAGAGAGGCGGGCCAGCACG25.0No Hit
CCAAGGCATTCACCTGCTCCAGGAGTGCAACCTGGGAAAGGATAAACTGC25.0No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA25.0Clontech Universal Primer Mix Long (95% over 21bp)
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG25.0No Hit
TATCAACGCAGAGTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACA12.5No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC12.5Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTGTGAGAGGTCGGGCCACGACCACGCAGCAATTGGAGAGGAACTGCA12.5No Hit
GTGCTCATTCTGAGTTTTGAAGTTTTGATGCACGGCAGTGCCACCTCCAT12.5No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTA12.5No Hit
GCATAGGCAAAGACTTCATGACTAAAACACCGAAAGCAGTATGGCAACAA12.5No Hit
TGCGGGAAGCGTGATACGCACTGCCCAGGTACAGAAAGGGCAGGATTTCC12.5No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.5Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTA12.5No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12.5Clontech Universal Primer Mix Long (96% over 26bp)
GATGATGATTCCATTTGTGTCCATTCGATGATTCCATTCAATTCCATCAG12.5No Hit
CCAGTGGATTCGGATGAAAACTGGAAATAAAATCAGGTACAACTCCAAAA12.5No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG12.5Clontech Universal Primer Mix Long (96% over 26bp)
GTACCGCTACGAATTCGTGCCCACGGCCAATGACAACGGCACCTACATCG12.5No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA12.5No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12.5Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAA12.5No Hit
CCCAAAGTGCTGGGATTATAGGCTTGAGCCACCTCACCTGGCCTAGTTTG12.5No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT12.5Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTCAATGATTCCATCAGATTCCATTTGATGATGATTCCATTCGATTC12.5No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.5Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAACTGTCTCTTATAC12.5No Hit
TTTTTTTGACGCTGCCGACGAACTGATTTTTGGTTTGAGTTCTAGGGTGT12.5No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)