FastQCFastQC Report
Tue 10 May 2022
EGAF00003626327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367639
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA31630.23127448105823245No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG29360.2146765337929088No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24180.17680104179538608No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23250.17000100172633276No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA22320.16320096165727943No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20400.14916216925665327No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20030.14645677697111592No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA19310.1411922298208811No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG18360.13424595233098793No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA18350.134172833620568No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC17910.13095561036209116No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16860.12327814576799873No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG16680.12196200898044No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG16240.11874478572196318No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16140.1180135986177639No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC16110.11779424248650411No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15820.11567379988432619No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT15670.11457701922802728No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC15150.11077484628619103No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT15150.11077484628619103No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT15100.11040925273409138No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC14810.10828881013191347No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG14610.1068264359235149No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA14450.10565653655679606No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT13960.10207371974621958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.10170812619411994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)