FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA198.15450643776824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC146.008583690987124Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125.150214592274678Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG62.575107296137339Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG62.575107296137339Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA52.1459227467811157Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52.1459227467811157Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT52.1459227467811157Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC52.1459227467811157Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG41.7167381974248928Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41.7167381974248928Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC31.2875536480686696Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31.2875536480686696Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.8583690987124464No Hit
GCTTGGGTCTGTAACAAAGCATTCATGTTTTAGAGCATAGGTCAGTAATT20.8583690987124464No Hit
CAGGTACATGGGAAGCAGTGGTATCAACGCAGGTACATGGGAAGCAGTGG20.8583690987124464Clontech Universal Primer Mix Long (95% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20.8583690987124464Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.8583690987124464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC20.8583690987124464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTCCGGCAGGGTCTTGCCCAGCTTGGCGACTTCATCAATGTCACCGG20.8583690987124464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.8583690987124464No Hit
ACCGTGATGAAGTCCGTCTTGTTGGCGGGCACCTGGCCATTGACGCGGCT20.8583690987124464No Hit
GCTGGAAGTACGCAGCAAAGCGGTGCAGGTACTGGTCATAGGGCAGCATG20.8583690987124464No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.8583690987124464Clontech Universal Primer Mix Long (95% over 22bp)
GTACCTTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA20.8583690987124464No Hit
GCAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.8583690987124464Clontech Universal Primer Mix Long (100% over 23bp)
GGTATCAACGCAGAGTACAGGGAAGCAGTGGTATCAACGCAGAGTACAGG20.8583690987124464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCTAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.8583690987124464Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.8583690987124464No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGCCCATGTACTCTGCGTTGA20.8583690987124464Clontech Universal Primer Mix Long (96% over 27bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.8583690987124464Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.8583690987124464Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTAAAAGGAAACACTTATAAA10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATAC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAGAA10.4291845493562232No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGAACTCTGC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGT10.4291845493562232Clontech Universal Primer Mix Long (96% over 27bp)
CAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GTGGGGTGGTGTGGATTCCGGGGAAGACACATACACACAGACTCTCCCGC10.4291845493562232No Hit
GTTGGCAGCATCACCTATCCATCATTATGAATTCGCTCTGCAATCGTCAT10.4291845493562232No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GATTCAGGTTTACTCACGTCATCCAGCAGAGAACGGAAAGTCAAATTTCC10.4291845493562232No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC10.4291845493562232No Hit
GCTGGCACGAAACCATTTTCGCCATCAGCCATGTAGAAGTAAGAACCACG10.4291845493562232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.4291845493562232No Hit
GGCCAATCCTGCATCTTTACACTTCTCCATGGCCTCCCATGTGGCACAGA10.4291845493562232No Hit
ATCATCGCCTACAAGCACGGCTACGAGGGCCTGCTCAAGGGAGACTTCCT10.4291845493562232No Hit
CATGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC10.4291845493562232Clontech SMART CDS Primer II A (96% over 26bp)
CCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GCAAGGGCCTGGTCACGCGCTCACTCCTTGATGTTGGCGGTCTGCACGAT10.4291845493562232No Hit
CTTCACATTCACATTGGCCCCAGCATCAATTAGAAGAGAGGCCACACTCT10.4291845493562232No Hit
ACATGGGGCAGTGGTATTCAACGCAAAAAAAAAAAAAAAAAAAAAGGACA10.4291845493562232No Hit
GCGGACAACTATGCTGAAAGCCAAGATCCTCTTCGTGGGGCCTTGCGAGA10.4291845493562232No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA10.4291845493562232No Hit
GATTTGGAGAAGCGGATGAAGTTCTCCTTTAGTTCCGAAGTCAGCTCCTT10.4291845493562232No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAAC10.4291845493562232No Hit
CCTTCTTCACACTGTAGCCAAACAAAGTGACTACAAGTCTGATCCCATCA10.4291845493562232No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGATAACACAGGA10.4291845493562232No Hit
GGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10.4291845493562232Clontech SMART CDS Primer II A (100% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 22bp)
TTCTAGGATAGTCAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGG10.4291845493562232No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATAAAGGCAAAAAAAAAA10.4291845493562232No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.4291845493562232Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAA10.4291845493562232No Hit
GTACATGGGAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232No Hit
TATCAACGCAGAGTACTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.4291845493562232No Hit
GCTGGAAGTACGCAGCAAAGCGGTGCGGGTACTGGTCATAGGGCAGCATG10.4291845493562232No Hit
ACGCAGAGAACGCGCCACATATCTTCTTCTGGGCTTTTCGGCTCGGCTTT10.4291845493562232No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCCTGTACCTGTCTCTTATAC10.4291845493562232Clontech Universal Primer Mix Long (96% over 28bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
TTCATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCAC10.4291845493562232No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG10.4291845493562232No Hit
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GGCATGCACCAAGAAACGAATGCCTGCAGCAAACAGGCGTCCTTGTATTC10.4291845493562232No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232No Hit
ACTGCAGCAGATGGGGCGGCGGCGGCTTCTACGCCATGGACTACTGTCTC10.4291845493562232No Hit
GTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGT10.4291845493562232No Hit
GTACATGCAAGCAGTGGTATCAACGCAGAGTACATGCAAGCAGTGGTATC10.4291845493562232Clontech Universal Primer Mix Long (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.4291845493562232Clontech SMART CDS Primer II A (100% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTATTCTGCGTTGAT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAAC10.4291845493562232No Hit
GTATAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACTGTCTCTTATAC10.4291845493562232No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CCTCAGCCTCTCAAAGTGCTGGGACTACAGGCGTGAGCCACCATGCCCAG10.4291845493562232No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA10.4291845493562232No Hit
TTCCCAGAGAATCTTCCAGTTGTACATCGTTCCACGTCGCTTCGACAACG10.4291845493562232No Hit
GTACATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAGTGG10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CTCTATACCATGACGCAAACGGACGATTTACTGCGCAAGCTGTACGATCA10.4291845493562232No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.4291845493562232Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GGCATGGATAGCACACAGGTTGGTGTCTTCAAAAAGGCCAACCAGATAGG10.4291845493562232No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGCTTCCCATGTACGCTGCGTT10.4291845493562232Clontech Universal Primer Mix Long (95% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCAATGTACTCTGCGTTGATACCAC10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 21bp)
ACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATTTAGACAACCCTTTATTTACAGGATTGCAGATAATTGTCTGACAAT10.4291845493562232No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGCAGTGGTA10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAGCAGTGGTAT10.4291845493562232Clontech SMART CDS Primer II A (100% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTACCACATCTTACC10.4291845493562232No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
GCCGCAGTTTCTGCGCGGCACACGGCTTTGGCAGCCCGGACAAGCCCGTA10.4291845493562232No Hit
AACCAGTGGCTTGGACAACCTGCAGGCCCGGGACTTTCTCGACGACCTCG10.4291845493562232No Hit
GTACATGGGGAGCGCCGCTCCGGCTGCACCGCGCTCGCTCCGAGTTTCAG10.4291845493562232No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 22bp)
TCCTTGCCTCGCTCACTGCACGCCAGAACCTGAGGAGAGAATGGCATGCC10.4291845493562232No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.4291845493562232Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGAGAAGCAGTGG10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
ACTTTGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT10.4291845493562232No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GCGGTGCGTAGGGACGCGCGCTGCGGGCTACGAGTCGGGTAGGGTGGTTG10.4291845493562232No Hit
ATGCTAAGATTTTGCGTAGCTGGGTTTGGTTTAATCCACCTCAACTGCCT10.4291845493562232No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGT10.4291845493562232No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232No Hit
TGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 21bp)
TCACTGGGCACTGCGAATGCCATGACTGAAGAATTAACAGCCACCCCTCA10.4291845493562232No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4291845493562232Clontech SMART CDS Primer II A (100% over 24bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACACTGCTTCCC10.4291845493562232Clontech Universal Primer Mix Long (95% over 21bp)

[FAIL]Adapter Content

Adapter graph